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Subject: search.filters.subject.Phytoremediation

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    The impact of nickel and chrome mine tailings on the growth of Hibiscus cannabinus and Linum usitatissimum and a preliminary assessment of their applicability as economically beneficial phytoremediation species
    (University of the Witwatersrand, Johannesburg, 2023-07) Campbell, Tiago Roberto; Furniss, David; Scholes, Mary
    Current and previous mining activities in South Africa have caused various environmental, human health and societal impacts. This has led to the formation and enforcement of legislation regarding the rehabilitation of active, closed and abandoned mines in South Africa. The requirements contained in this legislation include rehabilitation, skills transfer, job creation and development of post mine land use regarding active, closed and abandoned mines. A common impact of mining activities is the contamination of soils with various metals. The process of phytoremediation has demonstrated potential in the remediation of metal contaminated soils. Plant species commonly utilised in this process are hyper accumulators, which can translocate and accumulate high concentrations of various metals from soils into their biomass. However, large areas of previously economically productive land become underutilised when hyper-accumulators are used for phytoremediation. Economically valuable fibrous plant species have demonstrated potential in their use as phytoremediation species. This presents an opportunity in which economically valuable plant species could be utilised in phytoremediation applications on active, closed and abandoned mines in South Africa. Thus, the aim of this research was to assess the ability of Hibiscus cannabinus and Linum usitatissimum to grow in and extract metals from soil contaminated with nickel and chrome mine tailings. Furthermore, the concurrent use of H. cannabinus and L. usitatissimum as phytoremediative and economically beneficial plant species was determined. Normal (non impacted), rehabilitated (previously impacted) and tailings (impacted) soil treatments were collected and used from the Onverwacht tailings storage facility of Nkomati Nickel mine. Hibiscus cannabinus and L. usitatissimum were cultivated in each soil treatment in greenhouse conditions over a six-month period. Multiple plant growth parameters were recorded at monthly intervals. The amount (mg) and concentration (mg/kg) of Mn, Zn, Ni, Cu, Cr and Co contained within plant tissue samples at the end of the six-month period was determined. The area (ha) of land categories available for H. cannabinus and L. usitatissimum cultivation onsite was determined using Sentinel 2B satellite imagery and supervised image classification. The measured and expected total yield (t), yield value (R), profit/loss margin (R) and amount (g/ha) of Mn, Zn, Ni, Cu, Cr and Co extracted through cultivation of H. cannabinus and L. usitatissimum onsite was determined. The growth of H. cannabinus and L. usitatissimum cultivated in rehabilitated soil was severely impacted. While growth of each species exhibited minimal differences between those cultivated in normal and tailings soil. Hibiscus cannabinus consistently exhibited greater growth than L. usitatissimum. Both species demonstrated the ability to accumulate varying amounts and concentrations of each of the tested metals in their total, above and below ground components. Both species consistently accumulated increased amounts and concentrations of Mn and Zn. Those cultivated in tailings soil exhibited increased accumulation of Cr. Linum usitatissimum generally accumulated metals at higher concentrations than H. cannabinus, however, minimal differences in the amount of metal accumulated between species were observed. Based on the measured yield cultivation of each species onsite would result in economic loss and generally low metal extraction. However, based on the expected yield, species cultivation onsite, in normal and tailings soil, would result in economic gain and generally high metal extraction. Hibiscus cannabinus and L. usitatissimum exhibited phytoremediative and economic potential. Aspects of the current state of mine impacted land in South Africa and the requirements of rehabilitation enforced through South African legislation could possibly be addressed through the application of H. cannabinus and L. usitatissimum for mine rehabilitation strategies.
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    Comparative Analysis of Water Hyacinth Efficiency as Biosorbent and Phyto remediating Plant for Removal of Lead (Pb) Water Contaminants
    (University of the Witwatersrand, Johannesburg, 2023-08) Nwagbara, Victor Uzoma; Parrini, Francesca; Newete, Solomon
    One of the major problems facing the modern society is the issues of water contamination. The ability of aquatic plants to serve as both heavy metal biomarkers and phytoremediators has been advocated for many years. The purpose of this study was therefore to compare the efficacy of water hyacith as a biosorbent and phytoremediating plant for removal of lead from contaminated waters. Dry and fresh water hyacinth biomass were exposed to Pb-contaminated water at different time intervals of 1, 7, 14 and 21 days. The results showed that with the increase of the exposure time, the Pb removal capacity of the plant biomass also increased. The Pb water concentration in day 1 and day 7 were significantly different from those in day 14 and day 21 which were not significantly different from each other. The average Pb removal from the Pb-treated waters for the different time exposure of 1, 7, 14 and 21 days in the fresh water hyacinth biomass were 40%, 56%, 78 % and 79%, respectively, the highest reduction being in the latter. The biomass of the freshwater hyacinth roots had the highest Pd concentration compared to the shoot. On The other hand the average Pb removal by the dry water hyacinth biomass were 78.9 %, 78.5%, 78.3 % and 78.3% for day 1, 7, 14 and 21 respectively, showing no significant difference between the different time series exposure. This suggests the instant adsorption of Pb by the dry plant material from the first day of exposure, after which the active sites for adsorption saturates to accommodate any further Pb ion uptake. There were some plant stresses such as leaf chlorosis, and significant decrease in biomass weight and length of leaf-2 petiole in the lead-treated fresh water hyacinth plants compared to the controls (Pb-free water hyacinth plant). Thus, this study confirms that the largest removal by the dry water hyacinth biomass, occurred in the first day as opposed to the last day (day 21) by the freshwater hyacinth biomass.
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    Compost-assisted phytoremediation of mine tailings and footprint areas using chrysopogon zizanioides (l) roberty enhanced with moringa leaf extract biostimulant in the Witwatersrand goldfields of South Africa: a sustainability initiative
    (University of the Witwatersrand, Johannesburg, 2024) Mlalazi, Nkanyiso; Chimuka, Luke; Simatele, Mulala Danny
    In the Witwatersrand goldfields of South Africa, mine tailings and footprint areas are significant environmental problems because they are major sources of toxic metals. These metals can leach into soils, and both surface and ground water, causing serious risks to human, animal, and plant life. In this study, the compost-assisted phytoremediation of tailing storage facilities (TSFs) and footprint soil using Chrysopogon zizanioides (vetiver grass) enhanced with moringa leaf extract (MLE) was investigated. A greenhouse experiment was conducted to identify the most favorable parameters, and was followed by a field study to test the optimized parameters under real-environment settings. For the greenhouse experiment, a 3×2×2 fully crossed factorial design was used to determine the optimum variables. Vetiver growth was assessed under three compost concentrations (0%, 30% and 60%), two types of MLE (laboratory extracted MLE and commercial MLE) and two application regimens (once a week and twice a week) were used. The biomass and metal concentrations in the vetiver grass roots and leaves were measured after sixteen weeks followed by a two-way ANOVA analysis and the post-hoc tests. All the vetiver that was planted in 0% compost died within four weeks regardless of the MLE treatment. Vetiver grass planted on the 60% compost amendments and sprayed with laboratory extracted MLE had the highest biomass production, followed by plants grown in 30% compost amendments and sprayed with commercial biostimulant. However, the heavy metal removal or uptake data by the plant was inconclusive, as most of the toxic metals were not removed by vetiver grass which was attributed to the effect of compost. Based on biomass data, the 30% compost amendment and commercial bio-stimulant was the ideal treatments for the phytoremediation of gold mine tailings using vetiver grass. Although metal accumulation by plants is one of the attributes considered in phytoremediation, it is not the most significant factor in the phytostabilisation process. Plant growth and biomass production are the most significant, therefore it is concluded that vetiver, MLE and compost can be used in the phytostabilisation of gold mine tailings, however reduction in compost may be considered in future to improve the accumulation of metals in the roots for improved results. Following the conclusion of the greenhouse study, a field study was conducted during the rainy season of 2021. Two field experiments were carried out concurrently at two sites: the footprint area (that was used as a rock dump) and the tailings storage facility (TSF 4). A split-plot design was used in this study. The experiment at each site assumed a 3×1×2 factorial design, with three levels of compost treatment (0%, 15% and 30%), 1 level of vetiver cultivar (Chrysopogon zizanioides), and 2 levels of MLE treatment (commercial MLE and tap water, both sprayed once a week). Three blocks measuring 1 m × 2 m, each with 20 holes filled with equal amounts of soil amended with the different compost levels were prepared in triplicates. A single vetiver grass slip was planted in each hole. The blocks were then divided into 2 sections, each with 10 holes, and commercial MLE was sprayed on one section, while only water was sprayed on the other section once a week. After sixteen weeks, three plants were harvested from each section and the number of leaves, leaf length, number of tillers, biomass for roots and leaves and element concentrations were measured. Data analysis was done using two-way ANOVA