School of Oral Health Sciences

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Cemento-enamel junction variability within the mouth
(1988) Grossman, E. S.; Hargreaves, J. A.
Seal development and composition at amalgam-ceramic interfaces after NaCl and Na2S storage
(1987) Jodaikin, A.; Grossman, E. S.; Witcomb, M. J.
Base solubility and marginal sealing in amalgam restored teeth
(1991) Grossman, E. S.; Witcomb, M. J.; Matejka, J. M.
Marginal adaptation in composite resin restored dentine cavities Showing gross marginal leakage
(1986) Grossman, E. S.; Sparrius, O.
The aim of bonding composite resin restorations to the tooth is to ensure retention and also to prevent marginal leakage at the tooth restoration interface, a process which can lead to further caries. An in vitro marginal leakage study' of composite resins in combination wTEh acid' etching and dentine and enamel bonding agents highlighted the lower sealing potential of both enamel and dentine bonding agents to dentine (36%>seal) compared to enamel (79%>seal). Several studies 2-4 suggest that if the inner surface of the resin restoration appears as a well replicated negative impression of the adjacent tooth surface, bonding has been achieved. This study was undertaken to examine the appearance of the inner surface of resin restorations which showed gross marginal leakage in restored dentine cavities to determine the degree of marginal adaptation between the restoration and cavity surface.
A comparative sem study of keratinized and non-keratinized oral mucosa of the vervet monkey
(1981) Grossman, E. S.; Cleaton-Jones, P. E.
Scanning electron microscopy has revealed that the surfaces of keratinized and non-keratinized oral epithelial cells have characteristic features which enable these tissue types to be readily distinguished. These features include pits, villus-like projections and ridge-like folds called microplications. The origin and functional significance of these structures is not known, although several hypotheses have been proposed. Microplications have been interpreted as a reserve area for cell stretching but further studies have yielded conflicting results. Cellular interdigitation resulting from the microplications may aid adhesion between the stratified epithelial cells. This view has been supported by subsequent investigations of superficial epithelial cells. The situation in the deeper cell layers is not entirely clear.
Intercellular junctions in the attached gingiva and alveolar mucosa in the vervet monkey (cercopithecus aethiops)
(1979) Grossman, E. S.; Austin, J. C.; Cleaton-Jones, P.
This study was undertaken to determine the distribution of hemidesmosomes on the basal cell membranes adjacent to the basement lamina and the spinous cell membranes on unloaded keratinized AG and non- keratinized AM.
Analyses of marginal seals in aged amalgam restorations using extraction replicas
(1984) Grossman, E. S.; Witcomb, M. J.; Jodaikin, A
Since dental amalgam does not bond chemically to tooth structure, a microcrevice is present at the amalgam/tooth interface of a freshly placed restoration. This gap is of sufficient width to allow fluids and microorganisms to pass along the cavity walls and thereby facilitating the recurrence of dental caries, which accounts for the majority of amalgam restoration replacements.
Ultrastructural features of exfoliating surface cells in keratinised and non-keratinised oral mucosa
(1979) Austin, J. C.; Grossman, E. S.; Cleaton-Jones, P.
Healthy keratinised and non-keratinised oral mucosa (attached gingiva and alveolar mucosa) were obtained from 6 adult vervet monkeys, fixed in situ by simultaneous perfusion and immersion fixation using cacodylate buffered 2,5% glutaraldehyde and prepared for examination by SEM and TEM.
The effect of enamel etchants and a cleaning agent on cut dentine: an in vitro study
(1985) Sparrius, O.; Grossman, E. S.
Cavities 1,25 x 2,00mm were prepared in the cervical dentine of extracted human premolar teeth using a no. 577 tungsten carbide bur. The cavity depth was standardised at 1mm with the aid of a depth gauge fitted to the high speed airturbine. The cavities were hemisected with a low speed, water cooled, diamond disc saw, then cleaned with water and air. Thereafter the following solutions were applied to six cavities each as per manufacturers' instructions: Anhydron; Chemfill (Citric Acid); Enamelbond etchant and Scotchbond etchant gel. Once the cavities had been cleaned and dried, the specimens were prepared for scanning electron microscopy (SEM) and viewed at magnifications of x35, x200 and x2000. Cavity walls and floors were viewed separately and representative areas photographed. Similarly prepared unetched cavities served as a control.
Primary fixation of vervet monkey oral epithelium for SEM and TEM
(1987) Grossman, E. S.
Vervet monkey attached gingiva and alveolar mucosa was used to investigate the effect of primary fixative composition and osmolarity on the scanning electron microscope appearance and epithelial cell surface feature density. Primary fixation was obtained using 12 different fixatives with osmolarities varying between 320-2010mOsm followed by further standard SEM processing procedures. All primary fixatives investigated produced acceptably fixed oral epithelium for SEM study, showing all the morphologic features characteristic of either keratinized or non-keratinized oral tissue. Point counting revealed that the density of microvilli of attached gingiva epithelial cells when fixed at 2010mOsm was 72 +/- 8% of the cell surface area. This decreased to 40 +/- 5% when fixed at 320mOsm. Similarly the microplication density of the alveolar mucosa epithelial cells decreased from 70 +/- 5% at 2010mOsm to 43 +/- 7% at 320mOsm. Both these differences proved to be highly significant

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