Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes.

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dc.contributor.authorDuong, Y.T.
dc.contributor.authorKassanjee, R.
dc.contributor.authorWelte, A.
dc.contributor.authorAmpofo, W.
dc.contributor.authorParekh, B.S.
dc.contributor.authorMorgan, M.
dc.contributor.authorDe, A.
dc.contributor.authorDobbs, T.
dc.contributor.authorRottinghaus, E.
dc.contributor.authorNkengasong, J.
dc.contributor.authorCurlin, M.E.
dc.contributor.authorKittinunvorakoon, C.
dc.contributor.authorRaengsakulrach, B.
dc.contributor.authorMartin, M.
dc.contributor.authorChoopanya, K.
dc.contributor.authorVanichseni, S.
dc.contributor.authorJiang, Y.
dc.contributor.authorQiu, M.
dc.contributor.authorYu, H.
dc.contributor.authorHao, Y.
dc.contributor.authorShah, N.
dc.contributor.authorLe, L.-V.
dc.contributor.authorKim, A.A.
dc.contributor.authorNguyen, T.A.
dc.date.accessioned2016-07-19T14:34:40Z
dc.date.available2016-07-19T14:34:40Z
dc.date.issued2015-02-24
dc.descriptionArticleen_ZA
dc.description.abstractBackground: Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus. Methods: A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs. Results: Using different statistical methods,MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing formisclassification among long-terminfections indicated that overall PFRs were 0.6%to 2.5%at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C). Conclusions: Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use.en_ZA
dc.description.sponsorshipThis research has been supported by the President’s Emergency Plan for AIDS Relief (PEPFAR) through the Centers for Disease Control and Prevention (CDC).en_ZA
dc.identifier.citationDuong, Y.T. et al. 2015. Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes. PLoS ONE 10(2): e0114947.en_ZA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10539/20668
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.subjectantigenen_ZA
dc.subjectaccuracyen_ZA
dc.subjectacquired immune deficiency syndromeen_ZA
dc.subjectanalytical parametersen_ZA
dc.subjectantigen bindingen_ZA
dc.subjectcontrolled studyen_ZA
dc.subjectdisease durationen_ZA
dc.subjectenzyme immunoassayen_ZA
dc.subjecthumanen_ZA
dc.subjectHuman immunodeficiency virus 1en_ZA
dc.subjectHuman immunodeficiency virus 1 infectionen_ZA
dc.subjectlimiting antigen vidityen_ZA
dc.subjectmajor clinical studyen_ZA
dc.subjectmean duration of recent infectionen_ZA
dc.subjectproportion false recenten_ZA
dc.subjectReviewen_ZA
dc.subjectseroconversionen_ZA
dc.subjectstatistical analysisen_ZA
dc.subjectHIV infectionsen_ZA
dc.subjectBIOLOGICAL assayen_ZA
dc.subjectPARAMETER estimationen_ZA
dc.subjectLONGITUDINAL methoden_ZA
dc.subjectDATA analysisen_ZA
dc.titleRecalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes.en_ZA
dc.typeArticleen_ZA
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