The effects of lycopene on bone, kidney and pancreatic health of growing Wistar rats fed an obesogenic diet
| dc.contributor.author | Gomotsegang, Motlhale | |
| dc.date.accessioned | 2024-12-02T08:09:21Z | |
| dc.date.available | 2024-12-02T08:09:21Z | |
| dc.date.issued | 2024 | |
| dc.description | A dissertation submitted in fulfillment of the requirements for the degree of Master of Science in Medicine to the Faculty of Health Sciences, School of Physiology University of Witwatersrand, , Johannesburg 2024 | |
| dc.description.abstract | Dietary fructose causes obesity, oxidative stress, and inflammation that compromise bone, kidney, and pancreatic health. Lycopene, a phytochemical, has antioxidant and anti- inflammatory properties. Its potential prophylactic effects on diet-induced bone, kidney and pancreatic derangements were evaluated in growing Wistar rats fed a high-fructose diet. Ninety-six 21-day old Wistar rat pups (48 females, 48 males) randomly allocated to treatment groups: I - standard rat chow (SRC) + plain drinking water (PDW) + plain gelatine cube (PGC), II - SRC + 20% fructose as drinking water (FDW) + PGC, III - SRC + FDW + 100mg/kg body mass fenofibrate per day (FF), IV - SRC + FDW + 30mg/kg body mass lycopene per day and V - SRC + FDW + 60mg/kg body mass lycopene per day and VI - SRC + FDW + 100mg/kg body mass lycopene per day were fed for 84 days. Body, kidneys, pancreata, femora and tibiae masses were determined. Kidney and pancreatic lipid content, pancreatic TBARS concentration, SOD and GPX1 activities and plasma CTX-1 and osteocalcin concentrations were measured. Femora and tibia, length, mid-shaft, sub-trochanteric medio-lateral, head and neck transverse, medio-lateral, and epicondylar diameters, maximum distal epiphyseal and proximal epiphyseal breadths and breaking strength were measured. Treatments had no effect on rats’ pancreata and males’ kidney masses (P>0.05). Fenofibrate increased the females’ kidney masses (P = 0.0056). Rats’ tibiae and females’ femora masses were similar (P>0.05). CTX-1, osteocalcin and TBARS concentrations and SOD and GPX1 activities were similar (P>0.05). Treatments did not affect the rats’ kidney and males’ pancreatic lipid contents (P>0.05). Dietary fructose increased females’ pancreatic lipid content (P = 0.0048). Lycopene prevented the fructose-induced increased pancreatic lipid content (P = 0.0048). Rats’ tibiae and femora breaking strength and femora mid-shaft diameter (MIDD) of males were similar (P>0.05). Dietary fructose reduced the females’ MIDD and males’ sub-trochanteric medio- lateral diameter (STMLD) (P>0.05). Lycopene prevented the dietary fructose-induced MIDD decrease in females (P = 0.0003). Low dose lycopene decreased (P = 0.0003) the females’ STMLD. Dietary fructose mediated sexually dimorphic effects and supplemental lycopene can protect females against fructose-induced pancreatic lipid accretion and MIDD reduction and males against STMLD reduction. In females low dose lycopene may increase the risk of femora fracturing through reduced STMLD | |
| dc.description.submitter | MM2024 | |
| dc.faculty | Faculty of Health Sciences | |
| dc.identifier | https://orcid.org/ 0000-0002-0386-3894 | |
| dc.identifier.citation | Gomotsegang, Motlhale . (2024). The effects of lycopene on bone, kidney and pancreatic health of growing Wistar rats fed an obesogenic diet [Master’s dissertation, University of the Witwatersrand, Johannesburg]. WireDSpace.https://hdl.handle.net/10539/42993 | |
| dc.identifier.uri | https://hdl.handle.net/10539/42993 | |
| dc.language.iso | en | |
| dc.publisher | University of the Witwatersrand, Johannesburg | |
| dc.rights | © 2024 University of the Witwatersrand, Johannesburg. All rights reserved. The copyright in this work vests in the University of the Witwatersrand, Johannesburg. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of University of the Witwatersrand, Johannesburg. | |
| dc.rights.holder | University of the Witwatersrand, Johannesburg | |
| dc.school | School of Physiology | |
| dc.subject | lycopene | |
| dc.subject | High fructose diet | |
| dc.subject | Bone | |
| dc.subject | Kidney | |
| dc.subject | Pancreas | |
| dc.subject | Obesity | |
| dc.subject | Metabolic dysfunction | |
| dc.subject | UCTD | |
| dc.subject.other | SDG-3: Good health and well-being | |
| dc.title | The effects of lycopene on bone, kidney and pancreatic health of growing Wistar rats fed an obesogenic diet | |
| dc.type | Dissertation |