Developments in the use of pH and electric field strength gradients for the electrophoretic analysis of biomolecules
Date
2016-07-18
Authors
Bellini, Marco Paolo
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The electric field strength and pH gradients generated in isotachophoretic systems may be used for the separation of biomolecules. Poly (2 acrylamido 2-methyl-1-propanesulfonic acid) polymers of a uniform distribution of molecular mass were synthesized and used as spacers in isotachophoresis in order to generate linear electric field strength gradients in which biomolecules could be focused. These novel spacers are designed to operate within sieving media, and hence are useful for the separation of nucleic acids. [Abbreviated Abstract. Open document to view full version]
Description
A thesis submitted to the Faculty of Science, University
of the Witwatersrand, Johannesburg, in fulfilment of the
requirements for the degree of Doctor of Philosophy.
Johannesburg, 1996