Optimisation of expressed RNA interference mimics using predicted stem length

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dc.contributor.authorVan den Berg, Fiona Taylor
dc.date.accessioned2017-01-19T06:32:59Z
dc.date.available2017-01-19T06:32:59Z
dc.date.issued2016
dc.descriptionA thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy. Johannesburg, 2016.en_ZA
dc.description.abstractPrimary microRNA (pri-miRNA) mimics have been shown to mediate effective gene silencing and are well-suited for therapeutic applications. Pri-miRNA mimics, like natural pri-miRNA, are processed in the endogenous microRNA (miRNA) biogenesis pathway. Elements of the secondary RNA structure are crucial for processing by the Drosha-DGCR8 microprocessor, including a basal stem of - 11 bp. However, structural variation is common and the exact determinants of pri-miRNA processing have been elusive. The aim of this project were to explore the use of natural pri-miRNAs with exceptional basal stem in the design of correspondingmimics and to identify optimal stem features.[Abbreviated Abstract. Open document to view full version]en_ZA
dc.description.librarianLG2017en_ZA
dc.format.extentOnline resource (xv, 233 leaves)
dc.identifier.citationVan den Berg, Fiona Taylor (2016) Optimisation of expressed RNA interference mimics using predicted stem length, University of Witwatersrand, Johannesburg, <http://wiredspace.wits.ac.za/handle/10539/21678>
dc.identifier.urihttp://hdl.handle.net/10539/21678
dc.language.isoenen_ZA
dc.subject.lcshMicroRNA
dc.subject.lcshMicroRNA--Therapeutic use
dc.subject.lcshRNA
dc.titleOptimisation of expressed RNA interference mimics using predicted stem lengthen_ZA
dc.typeThesisen_ZA
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