Optimisation of expressed RNA interference mimics using predicted stem length
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Date
2016
Authors
Van den Berg, Fiona Taylor
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Abstract
Primary microRNA (pri-miRNA) mimics have been shown to mediate effective gene silencing and are well-suited for therapeutic applications. Pri-miRNA mimics, like natural pri-miRNA, are processed in the endogenous microRNA (miRNA) biogenesis pathway. Elements of the secondary RNA structure are crucial for processing by the Drosha-DGCR8 microprocessor, including a basal stem of - 11 bp. However, structural variation is common and the exact determinants of pri-miRNA processing have been elusive. The aim of this project were to explore the use of natural pri-miRNAs with exceptional basal stem in the design of correspondingmimics and to identify optimal stem features.[Abbreviated Abstract. Open document to view full version]
Description
A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy. Johannesburg, 2016.
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Van den Berg, Fiona Taylor (2016) Optimisation of expressed RNA interference mimics using predicted stem length, University of Witwatersrand, Johannesburg, <http://wiredspace.wits.ac.za/handle/10539/21678>