Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome
|Belotserkovsky, Jaroslav Michailovich
|Master of Science - Adult Education
|Mutations were sought in Escherichia coli ribosomal RNA and ribosomal proteins that confer dependence to the antibiotic streptomycin, using both newly available as well as well-established genetic systems. I found that a classical ribosomal mutant, Sm-D3, was streptomycin dependent and had an additional mutation in another ribosomal component – protein L7/L12. The double mutant had an 8-fold lower streptomycin requirement as compared to Sm-D3 with a wild-type rplL. This supported a functional involvement of L7/L12 in the decoding center of the ribosome.
|Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome