Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome
| dc.contributor.author | Belotserkovsky, Jaroslav Michailovich | |
| dc.date.accessioned | 2006-02-08T13:45:45Z | |
| dc.date.available | 2006-02-08T13:45:45Z | |
| dc.date.issued | 2006-02-08 | |
| dc.description | Master of Science - Adult Education | en |
| dc.description.abstract | Mutations were sought in Escherichia coli ribosomal RNA and ribosomal proteins that confer dependence to the antibiotic streptomycin, using both newly available as well as well-established genetic systems. I found that a classical ribosomal mutant, Sm-D3, was streptomycin dependent and had an additional mutation in another ribosomal component – protein L7/L12. The double mutant had an 8-fold lower streptomycin requirement as compared to Sm-D3 with a wild-type rplL. This supported a functional involvement of L7/L12 in the decoding center of the ribosome. | en |
| dc.format.extent | 1152370 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10539/164 | |
| dc.language.iso | en | |
| dc.subject | escherichia | en |
| dc.subject | coli | en |
| dc.subject | ribosome | en |
| dc.subject | mutational | en |
| dc.title | Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome | en |
| dc.type | Thesis | en |