Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome
Date
2006-02-08
Authors
Belotserkovsky, Jaroslav Michailovich
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Abstract
Mutations were sought in Escherichia coli ribosomal RNA and ribosomal proteins
that confer dependence to the antibiotic streptomycin, using both newly available as well as well-established genetic systems. I found that a classical ribosomal mutant, Sm-D3, was streptomycin dependent and had an additional mutation in another ribosomal component – protein L7/L12. The double mutant had an 8-fold lower streptomycin requirement as compared to Sm-D3 with a wild-type rplL. This supported a functional involvement of L7/L12 in the decoding center of the ribosome.
Description
Master of Science - Adult Education
Keywords
escherichia, coli, ribosome, mutational