Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves

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dc.contributor.author T Sebola
dc.contributor.author N Uche-Okereafor
dc.contributor.author L Mekuto
dc.contributor.author Maya Makatini
dc.contributor.author E Green
dc.contributor.author Vuyo Mavumengwana
dc.date.accessioned 2021-04-19T10:38:04Z
dc.date.available 2021-04-19T10:38:04Z
dc.date.issued 2020-12-10
dc.identifier.citation SCOPUS en_ZA
dc.identifier.issn 1687918X
dc.identifier.issn Electronic: 1687-9198
dc.identifier.uri https://hdl.handle.net/10539/30900
dc.description.abstract This study isolated and identified endophytic bacteria from the leaves of Crinum macowanii and investigated the potential of the bacterial endophyte extracts as antibacterial and anticancer agents and their subsequent secondary metabolites. Ethyl acetate extracts from the endophytes and the leaves (methanol: dichloromethane (1 : 1)) were used for antibacterial activity against selected pathogenic bacterial strains by using the broth microdilution method. The anticancer activity against the U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Bacterial endophytes that were successfully isolated from C. macowanii leaves include Raoultella ornithinolytica, Acinetobacter guillouiae, Pseudomonas sp., Pseudomonas palleroniana, Pseudomonas putida, Bacillus safensis, Enterobacter asburiae, Pseudomonas cichorii, and Arthrobacter pascens. Pseudomonas cichorii exhibited broad antibacterial activity against both Gram-negative and Gram-positive pathogenic bacteria while Arthrobacter pascens displayed the least MIC of 0.0625 mg/mL. Bacillus safensis crude extracts were the only sample that showed notable cell reduction of 50% against A549 lung carcinoma cells at a concentration of 100 mu g/mL. Metabolite profiling of Bacillus safensis, Pseudomonas cichorii, and Arthrobacter pascens crude extracts revealed the presence of known antibacterial and/or anticancer agents such as lycorine (1), angustine (2), crinamidine (3), vasicinol (4), and powelline. It can be concluded that the crude bacterial endophyte extracts obtained from C. macowanii leaves can biosynthesize bioactive compounds and can be bioprospected for medical application into antibacterial and anticancer agents. en_ZA
dc.language.iso en en_ZA
dc.publisher Hindawi en_ZA
dc.rights Copyright © 2020 Creative Commons Attribution License en_ZA
dc.subject FUNGAL ENDOPHYTES en_ZA
dc.subject MEDICINAL PLANTS en_ZA
dc.subject IN-VITRO en_ZA
dc.subject NATURAL PRODUCTS en_ZA
dc.subject IDENTIFICATION en_ZA
dc.subject INSIGHTS en_ZA
dc.subject PHARMACOLOGY en_ZA
dc.subject PHYTOCHEMISTRY en_ZA
dc.subject ALKALOIDS en_ZA
dc.subject ANTIFUNGAL en_ZA
dc.title Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves en_ZA
dc.type Article en_ZA
dc.journal.volume 2020 en_ZA
dc.journal.title INTERNATIONAL JOURNAL OF MICROBIOLOGY en_ZA
dc.description.librarian NLB 2021 en_ZA
dc.citation.doi 10.1155/2020/8839490 en_ZA
dc.citation.epage 15
dc.citation.spage 1
dc.funder CSIR DST-Interbursary Support (IBS) bursary University of Johannesburg National Research Foundation - South Africa en_ZA
dc.orcid.id http://orcid.org/0000-0002-4962-0724 en_ZA
dc.journal.issue Article Number: 8839490 en_ZA
dc.article.start-page 1 en_ZA
dc.article.end-page 15 en_ZA
dc.faculty Faculty of Science en_ZA
dc.school Chemistry en_ZA


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