Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves

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dc.article.end-page15en_ZA
dc.article.start-page1en_ZA
dc.citation.doi10.1155/2020/8839490en_ZA
dc.citation.epage15
dc.citation.spage1
dc.contributor.authorT Sebola
dc.contributor.authorN Uche-Okereafor
dc.contributor.authorL Mekuto
dc.contributor.authorMaya Makatini
dc.contributor.authorE Green
dc.contributor.authorVuyo Mavumengwana
dc.date.accessioned2021-04-19T10:38:04Z
dc.date.available2021-04-19T10:38:04Z
dc.date.issued2020-12-10
dc.description.abstractThis study isolated and identified endophytic bacteria from the leaves of Crinum macowanii and investigated the potential of the bacterial endophyte extracts as antibacterial and anticancer agents and their subsequent secondary metabolites. Ethyl acetate extracts from the endophytes and the leaves (methanol: dichloromethane (1 : 1)) were used for antibacterial activity against selected pathogenic bacterial strains by using the broth microdilution method. The anticancer activity against the U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Bacterial endophytes that were successfully isolated from C. macowanii leaves include Raoultella ornithinolytica, Acinetobacter guillouiae, Pseudomonas sp., Pseudomonas palleroniana, Pseudomonas putida, Bacillus safensis, Enterobacter asburiae, Pseudomonas cichorii, and Arthrobacter pascens. Pseudomonas cichorii exhibited broad antibacterial activity against both Gram-negative and Gram-positive pathogenic bacteria while Arthrobacter pascens displayed the least MIC of 0.0625 mg/mL. Bacillus safensis crude extracts were the only sample that showed notable cell reduction of 50% against A549 lung carcinoma cells at a concentration of 100 mu g/mL. Metabolite profiling of Bacillus safensis, Pseudomonas cichorii, and Arthrobacter pascens crude extracts revealed the presence of known antibacterial and/or anticancer agents such as lycorine (1), angustine (2), crinamidine (3), vasicinol (4), and powelline. It can be concluded that the crude bacterial endophyte extracts obtained from C. macowanii leaves can biosynthesize bioactive compounds and can be bioprospected for medical application into antibacterial and anticancer agents.en_ZA
dc.description.librarianNLB 2021en_ZA
dc.facultyFaculty of Scienceen_ZA
dc.funderCSIR DST-Interbursary Support (IBS) bursary University of Johannesburg National Research Foundation - South Africaen_ZA
dc.identifier.citationSCOPUSen_ZA
dc.identifier.issn1687918X
dc.identifier.issnElectronic: 1687-9198
dc.identifier.urihttps://hdl.handle.net/10539/30900
dc.journal.issueArticle Number: 8839490en_ZA
dc.journal.titleINTERNATIONAL JOURNAL OF MICROBIOLOGYen_ZA
dc.journal.volume2020en_ZA
dc.language.isoenen_ZA
dc.orcid.idhttp://orcid.org/0000-0002-4962-0724en_ZA
dc.publisherHindawien_ZA
dc.rightsCopyright © 2020 Creative Commons Attribution Licenseen_ZA
dc.schoolChemistryen_ZA
dc.subjectFUNGAL ENDOPHYTESen_ZA
dc.subjectMEDICINAL PLANTSen_ZA
dc.subjectIN-VITROen_ZA
dc.subjectNATURAL PRODUCTSen_ZA
dc.subjectIDENTIFICATIONen_ZA
dc.subjectINSIGHTSen_ZA
dc.subjectPHARMACOLOGYen_ZA
dc.subjectPHYTOCHEMISTRYen_ZA
dc.subjectALKALOIDSen_ZA
dc.subjectANTIFUNGALen_ZA
dc.titleAntibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leavesen_ZA
dc.typeArticleen_ZA
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