Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18.

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dc.contributor.author Khumalo, T.
dc.contributor.author Reusch, U.
dc.contributor.author Knackmuss, S.
dc.contributor.author Little, M.
dc.contributor.author Veale, R.B.
dc.contributor.author Weiss, S.F.T.
dc.date.accessioned 2016-10-17T10:19:36Z
dc.date.available 2016-10-17T10:19:36Z
dc.date.issued 2013-06-18
dc.identifier.citation Khumalo, T. et al. 2013. Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18. PLoS ONE 8(6): e66297. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10539/21212
dc.description.abstract Adhesion and invasion have been identified as the two key components of metastasis. The 37 kDa/67 kDa laminin receptor (LRP/LR) is thought to enhance these two processes thus endorsing the progression of cancer. Here we report on LRP/LR and the metastatic potential of MDA-MB 231 breast and WHCO1 oesophageal cancer cells. Western blot analysis revealed a significant increase in total laminin receptor precursor (LRP) levels of breast and oesophageal cancer cells in comparison to non-invasive MCF-7 breast cancer cells, whereas LRP/LR cell surface levels in both cell lines were not significantly different to those of MCF-7 cells as analysed by flow cytometry. Incubation of breast and oesophageal cancer cells with the anti-LRP/LR specific antibody, IgG1-iS18, resulted in significant reduction in the adhesive potential of WHCO1 and MDA-MB 231 cells by 92% and 16%, respectively. Moreover, invasion was significantly impeded by 98% and 25% for WHCO1 and MDA-MB 231 cells, respectively. Pearson's correlation coefficients proved a positive correlation between total LRP/LR levels and invasive potential as well as between the adhesive and invasive potential of breast and oesophageal cancer cells. Our findings suggest that through interference of the LRP/LR-laminin-1 interaction, anti-LRP/LR specific antibody IgG1-iS18 may act as a possible alternative therapeutic tool for metastatic breast and oesophageal cancer treatment. en_ZA
dc.description.sponsorship This work was supported by the National Research Foundation, the Republic of South Africa and the Medical Research Council, the Republic of South Africa. en_ZA
dc.language.iso en en_ZA
dc.publisher Public Library of Science. en_ZA
dc.rights © 2013 Khumalo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.subject immunoglobulin G1 iS18 en_ZA
dc.subject laminin 1 en_ZA
dc.subject laminin receptor en_ZA
dc.subject receptor antibody en_ZA
dc.subject unclassified drug en_ZA
dc.subject breast cancer en_ZA
dc.subject cell adhesion en_ZA
dc.subject cell invasion en_ZA
dc.subject cell strain MCF 7 en_ZA
dc.subject cell strain MDA MB 231 en_ZA
dc.subject cell strain WHCO1 en_ZA
dc.subject WHCO1 en_ZA
dc.subject cancer cell en_ZA
dc.title Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18. en_ZA
dc.type Article en_ZA
dc.journal.volume 8 en_ZA
dc.journal.title PLoS ONE en_ZA
dc.description.librarian NCS2016. en_ZA
dc.citation.doi 10.1371/journal.pone.0066297. en_ZA
dc.citation.issue 6 en_ZA


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