Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18.

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dc.citation.doi10.1371/journal.pone.0066297.en_ZA
dc.citation.issue6en_ZA
dc.contributor.authorKhumalo, T.
dc.contributor.authorReusch, U.
dc.contributor.authorKnackmuss, S.
dc.contributor.authorLittle, M.
dc.contributor.authorVeale, R.B.
dc.contributor.authorWeiss, S.F.T.
dc.date.accessioned2016-10-17T10:19:36Z
dc.date.available2016-10-17T10:19:36Z
dc.date.issued2013-06-18
dc.description.abstractAdhesion and invasion have been identified as the two key components of metastasis. The 37 kDa/67 kDa laminin receptor (LRP/LR) is thought to enhance these two processes thus endorsing the progression of cancer. Here we report on LRP/LR and the metastatic potential of MDA-MB 231 breast and WHCO1 oesophageal cancer cells. Western blot analysis revealed a significant increase in total laminin receptor precursor (LRP) levels of breast and oesophageal cancer cells in comparison to non-invasive MCF-7 breast cancer cells, whereas LRP/LR cell surface levels in both cell lines were not significantly different to those of MCF-7 cells as analysed by flow cytometry. Incubation of breast and oesophageal cancer cells with the anti-LRP/LR specific antibody, IgG1-iS18, resulted in significant reduction in the adhesive potential of WHCO1 and MDA-MB 231 cells by 92% and 16%, respectively. Moreover, invasion was significantly impeded by 98% and 25% for WHCO1 and MDA-MB 231 cells, respectively. Pearson's correlation coefficients proved a positive correlation between total LRP/LR levels and invasive potential as well as between the adhesive and invasive potential of breast and oesophageal cancer cells. Our findings suggest that through interference of the LRP/LR-laminin-1 interaction, anti-LRP/LR specific antibody IgG1-iS18 may act as a possible alternative therapeutic tool for metastatic breast and oesophageal cancer treatment.en_ZA
dc.description.librarianNCS2016.en_ZA
dc.description.sponsorshipThis work was supported by the National Research Foundation, the Republic of South Africa and the Medical Research Council, the Republic of South Africa.en_ZA
dc.identifier.citationKhumalo, T. et al. 2013. Adhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18. PLoS ONE 8(6): e66297.en_ZA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10539/21212
dc.journal.titlePLoS ONEen_ZA
dc.journal.volume8en_ZA
dc.language.isoenen_ZA
dc.publisherPublic Library of Science.en_ZA
dc.rights© 2013 Khumalo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.subjectimmunoglobulin G1 iS18en_ZA
dc.subjectlaminin 1en_ZA
dc.subjectlaminin receptoren_ZA
dc.subjectreceptor antibodyen_ZA
dc.subjectunclassified drugen_ZA
dc.subjectbreast canceren_ZA
dc.subjectcell adhesionen_ZA
dc.subjectcell invasionen_ZA
dc.subjectcell strain MCF 7en_ZA
dc.subjectcell strain MDA MB 231en_ZA
dc.subjectcell strain WHCO1en_ZA
dc.subjectWHCO1en_ZA
dc.subjectcancer cellen_ZA
dc.titleAdhesion and Invasion of Breast and Oesophageal Cancer Cells Are Impeded by Anti-LRP/LR-Specific Antibody IgG1-iS18.en_ZA
dc.typeArticleen_ZA
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