School of Animal, Plant and Environmental Sciences (ETDs)

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Subject: search.filters.subject.SDG-17: Partnerships for the goals

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    Influence of polyploidy on morphology, genetic differentiation and reproductive strategy amongst varieties of Rhodohypoxis baurii (Hypoxidaceae)
    (University of the Witwatersrand, Johannesburg, 2023) Ferreira, Bianca Tasha; Goodman-Cron, Glynis V.; Glennon, Kelsey L.
    Evolutionary mechanisms, such polyploidy (increase in chromosome sets), alters plant morphology, gene flow and reproductive strategies, which can facilitate the generation or loss of species. Rhodohypoxis L. (Hypoxidaceae) is a small near-endemic Drakensberg genus comprising six species, one of which is Rhodohypoxis baurii. Rhodohypoxis baurii contains three morphologically distinct varieties, with varying ploidy-levels: R. baurii var. baurii (2×, 4×), R. baurii var. platypetala (2× 3× , 4×), and R. baurii var. confecta (2×). Therefore, R. baurii is an ideal system to evaluate whether polyploidy leads to lineage divergence or homogenization and contributes to biodiversity in this lineage. The aim of the study was to assess the influence of ploidy on morphology, genetic differentiation and reproductive strategy among varieties of Rhodohypoxis baurii (Hypoxidaceae), as well as to better recognise the three varieties. Thirty vegetative, floral, and reproductive traits were measured across 124 herbarium specimens and 43 individuals housed in the greenhouse. A matrix containing 20 quantitative and 12 qualitative characters was constructed and a Principal Coordinates Analysis (PCoA) and Non-Metric Multidimensional Scaling analysis (NMDS) were conducted. Important distinguishing morphological features that had a high eigenvalue (shown via a PCA) were selected for direct comparison using box and whisker box plots to compare means ± standard errors (SE). Certain morphological traits such as anther length, peduncle length and tepal sizes differed significantly among the varieties and ploidy levels, with polyploid individuals exhibiting the gigas effect. This was especially evident in R. baurii var. platypetala, which contained many polyploid individuals and exhibited larger flowers (longer and wider tepals) and larger anthers compared to other varieties. Rhodohypoxis baurii var. confecta and R. baurii var. platypetala are genetically, geographically and morphologically similar, differing only in flower colour, flower size and peduncle length. However, most of these differences can be attributed to differences in ploidy and /or altitude with R. baurii var. confecta occurring at higher altitudes and R. baurii var. platypetala containing multiple ploidy-levels. It is therefore evident that ecological differences and polyploidy have shaped the morphological differences in these two taxa. In addition, R. baurii var. baurii populations in the 7 Eastern Cape Drakensberg were morphologically, geographically and genetically distinct from all other populations and varieties, and may be a new/ undescribed taxon; however, this warrants further investigation. Out of 231 experimental crosses, 113 intra-varietal and inter-varietal crosses produced seeds. Rhodohypoxis baurii polyploid individuals show a shift away from sexual reproduction to asexual reproduction as they all showed higher rates of clonal reproduction than the diploid individuals. Moreover, crosses between polyploids yielded lower seed sets and lower germination rates than diploid-diploid crosses. Genetic differentiation and gene flow were quantified for 280 individuals among the varieties and ploidy-levels across 11 populations using 12 microsatellite markers labelled with the FAM NED dyes. Leaf material was collected from 237 individuals of Rhodohypoxis baurii (R. baurii var. confecta n = 88, R. baurii var. baurii n = 87, R. baurii var. platypetala n = 62) and flow cytometry conducted to estimate ploidy. A latitudinal ploidy gradient was evident across sampled populations that corresponds with shifts in reproductive strategy, and changes in the extent of gene flow. Population genetic structure coincided primarily with geographic localities, with diploid Northern Drakensberg populations having similar allelic diversity to one another. The Central and Southern Drakensberg mixed ploidy populations also showed similar allelic diversity but differed from the tetraploid Eastern Cape Drakensberg populations. Furthermore, gene flow was higher between geographically close populations irrespective of ploidy-level, with geographically isolated regions (such as the Eastern Cape Drakensberg) and outlying populations (i.e. Karkloof) showing unique genotypes, indicating little gene flow and allele sharing. Consequently, shifts in reproductive strategy and geographic isolation are likely changing gene flow patterns among varieties and ploidy levels which appears to be facilitating both lineage diversification and homogenization in this species
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    The reporting of the sustainability goals and the Sustainable Development Goals (SDGs) in the context of the private sector in South Africa
    (University of the Witwatersrand, Johannesburg, 2022) Mosi, Tumelo
    Sustainability challenges continue to mount and the fate of this planet lies in a precarious position. In response, the United Nations has introduced a global agreement known as the Sustainable Development Goals (SDGs). These are 17 goals underpinned by 169 targets for addressing social, economic and environmental issues on a global scale. The SDGs seek to do so by unlocking the potential that lies within the private sector and more. The aim of my research was to assess the sustainability goals disclosed by the top 25 Johannesburg Stock Exchange (JSE) listed companies against that of five other countries in 2017. Furthermore, I compared the environmental SDG disclosure of five companies from the metals and mining sector as well as the consumer products sector respectively, within the same period. The sample was selected from the original sample of the top 25 JSE listed companies. Comparisons were done by analysing sustainability/annual/integrated reports of the top 25 JSE listed companies. The results for the sample showed South Africa to be lagging behind the other countries with regards to disclosing sustainability goals in the corporate sustainability reporting for 2017. From 2017 to 2019, the number of reports addressing environmental SDGs from the selected sectors showed an increase. Furthermore, the study companies from the metal and mining sector performed better than those from the consumer products sector with regards to environmental SDG disclosure. However, the environmental SDG score of the study companies from the consumer products sector increased relatively more within the three years of assessment (2017-2019). Variation was also found between the study companies from the two sectors with regards to preference for specific environmental SDGs which included Responsible Consumption and Production (SDG 12), Climate Action (SDG 13), Life Below Water (SDG 14) and Life on Land (SDG 15). Moreover, the iii interviews conducted with the relevant participants revealed that SDG awareness has improved overtime, however internalisation of the SDGs remains the biggest challenge.