Wits Research Outputs
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Browsing Wits Research Outputs by Faculty "Faculty of Science"
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Item Antibacterial and Anticancer Activity and Untargeted Secondary Metabolite Profiling of Crude Bacterial Endophyte Extracts from Crinum macowanii Baker Leaves(Hindawi, 2020-12-10) T Sebola; N Uche-Okereafor; L Mekuto; Maya Makatini; E Green; Vuyo MavumengwanaThis study isolated and identified endophytic bacteria from the leaves of Crinum macowanii and investigated the potential of the bacterial endophyte extracts as antibacterial and anticancer agents and their subsequent secondary metabolites. Ethyl acetate extracts from the endophytes and the leaves (methanol: dichloromethane (1 : 1)) were used for antibacterial activity against selected pathogenic bacterial strains by using the broth microdilution method. The anticancer activity against the U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Bacterial endophytes that were successfully isolated from C. macowanii leaves include Raoultella ornithinolytica, Acinetobacter guillouiae, Pseudomonas sp., Pseudomonas palleroniana, Pseudomonas putida, Bacillus safensis, Enterobacter asburiae, Pseudomonas cichorii, and Arthrobacter pascens. Pseudomonas cichorii exhibited broad antibacterial activity against both Gram-negative and Gram-positive pathogenic bacteria while Arthrobacter pascens displayed the least MIC of 0.0625 mg/mL. Bacillus safensis crude extracts were the only sample that showed notable cell reduction of 50% against A549 lung carcinoma cells at a concentration of 100 mu g/mL. Metabolite profiling of Bacillus safensis, Pseudomonas cichorii, and Arthrobacter pascens crude extracts revealed the presence of known antibacterial and/or anticancer agents such as lycorine (1), angustine (2), crinamidine (3), vasicinol (4), and powelline. It can be concluded that the crude bacterial endophyte extracts obtained from C. macowanii leaves can biosynthesize bioactive compounds and can be bioprospected for medical application into antibacterial and anticancer agents.Item Biological and geophysical feedbacks with fire in the Earth system(Environmental Research Letters, 2018-03-06) Archibald, S.; Lehmann, C.E.R.; Belcher, C.M.; Bond, W.J.; Bradstock, R.A.Roughly 3% of the Earth's land surface burns annually, representing a critical exchange of energy and matter between the land and atmosphere via combustion. Fires range from slow smouldering peat fires, to low-intensity surface fires, to intense crown fires, depending on vegetation structure, fuel moisture, prevailing climate, and weather conditions. While the links between biogeochemistry, climate and fire are widely studied within Earth system science, these relationships are also mediated by fuels-namely plants and their litter-that are the product of evolutionary and ecological processes. Fire is a powerful selective force and, over their evolutionary history, plants have evolved traits that both tolerate and promote fire numerous times and across diverse clades. Here we outline a conceptual framework of how plant traits determine the flammability of ecosystems and interact with climate and weather to influence fire regimes. We explore how these evolutionary and ecological processes scale to impact biogeochemical and Earth system processes. Finally, we outline several research challenges that, when resolved, will improve our understanding of the role of plant evolution in mediating the fire feedbacks driving Earth system processes. Understanding current patterns of fire and vegetation, as well as patterns of fire over geological time, requires research that incorporates evolutionary biology, ecology, biogeography, and the biogeosciences.Item Carbon Encapsulated Ternary MnNiCo Oxide Nanoparticles as Electrode Materials for Energy Storage Applications(Wiley, 2020-12) Tarekegn Heliso Dolla; Isiaka A. Lawal; David Billing; K Pruessner; Patrick Ndungu; Dolla, TH; Lawal, IA; Billing, DG; Pruessner, k; Ndungu, PTernary transition metal oxides are promising advanced materials for use as electrode components in electrochemical energy storage systems. However, low electronic/ionic conductivity hinder practical applications. In this study, ternary Mn-Ni-Co oxide nanoparticles were encapsulated in carbon nanosheets, to improve the electrical conductivity and surface area. When tested as supercapacitor electrodes, the materials exhibited specific capacity of 91.2 mAh g(-1)at a current density of 1 A g(-1)in 2 M KOH. Moreover, after 3000 cycles the composite achieved a specific capacity of 74.6 mAh g(-1)at a current density of 6 A g(-1)and high capacitance retention of 96.4 %.Item Carnivore conservation needs evidence based livestock protection(Public Library of Science, 2018-09) van Eeden, L.M.; Eklund, A.; Miller, J.R.B.; López-Bao, J.V; Chapron, G.; Cejtin, M.R.; McManus, J.Carnivore predation on livestock often leads people to retaliate. Persecution by humans has contributed strongly to global endangerment of carnivores. Preventing livestock losses would help to achieve three goals common to many human societies: preserve nature, protect animal welfare, and safeguard human livelihoods. Between 2016 and 2018, four independent reviews evaluated >40 years of research on lethal and nonlethal interventions for reducing predation on livestock. From 114 studies, we find a striking conclusion: scarce quantitative comparisons of interventions and scarce comparisons against experimental controls preclude strong inference about the effectiveness of methods. For wise investment of public resources in protecting livestock and carnivores, evidence of effectiveness should be a prerequisite to policy making or large-scale funding of any method or, at a minimum, should be measured during implementation. An appropriate evidence base is needed, and we recommend a coalition of scientists and managers be formed to establish and encourage use of consistent standards in future experimental evaluations.Item Chiefs in a Democracy: A Case Study of the 'New' Systems of Regulating Firewood Harvesting in Post-Apartheid South Africa(MDPI, 2018-03) Findlay, S.J.; Twine, W.C.Much of the international commons literature reveals a decreased functioning of local traditional institutions that regulate natural resource harvesting. In South Africa, it is believed that the creation of new democratic structures at the end of Apartheid has contributed significantly to the deterioration in traditional resource regulation and this in turn has led to the extensive resource degradation seen in parts of the country. Many of these assertions, though, remain anecdotal in nature. Given the high reliance by rural households on natural resources, and the serious negative implications that over-use has on livelihood security, understanding how well or poorly such commons are regulated is key to ensuring the sustainability of such resource-dependent populations. The aim of this study was therefore to examine systems of resource governance, focusing specifically on firewood, and to determine the roles of traditional and democratically elected community leaders in six rural villages spanning two chieftaincies in Bushbuckridge, South Africa. In each study village, five local leaders were interviewed and five community focus groups were conducted. Results indicate that most parties still regard the Chief as the ultimate authority for regulating firewood harvesting. However, overall firewood management appears weak, at best, across the region. Although some authors attribute this to community confusion over the roles of local leaders in a new democracy, we provide evidence that other socio-political factors, including political expediency, may be driving the increasingly relaxed implementation of these firewood management systems. With resource dependence remaining a vital contributor to livelihood security across the developing world and with many rural communities facing increasing strain under local resource depletion, these findings shed new light on the complex social dynamics underlying the widely reported weakening of traditional institutions in South Africa. In so doing, it offers insights into local firewood governance that can be used to combat these challenges and thereby reduce regional social and ecological vulnerability being experienced in communal landscapes across the region.Item Forward scattering on the line with a transfer condition(SpringerOpen [Commercial Publisher], 2013-12) Currie, Sonja; Nowaczyk, Marlena; Watson, Bruce A.We consider scattering on the line with a transfer condition at the origin. Under suitable growth conditions on the potential, the spectrum consists of a finite number of eigenvalues which are negative real numbers, while the remainder is continuous spectrum which is comprised of the positive real axis. Asymptotics are provided for the Jost solutions. Conditions which characterize transfer conditions resulting in self-adjoint problems are found. Properties are given of the scattering coefficient linking it to the spectrum.Item Ivo Hummelgen(Springer, 2019-11-09) Neil Coville; Coville, N.Item A metagenomic viral discovery approach identifies potential zoonotic and novel mammalian viruses in Neoromicia bats within South Africa(Public Library of Science, 2018-03) Geldenhuys, M.; Mortlock, M.; Weyer, J.; Bezuidt, O.; Seamark, E.C.J.; Kearney, T.Species within the Neoromicia bat genus are abundant and widely distributed in Africa. It is common for these insectivorous bats to roost in anthropogenic structures in urban regions. Additionally, Neoromicia capensis have previously been identified as potential hosts for Middle East respiratory syndrome (MERS)-related coronaviruses. This study aimed to ascertain the gastrointestinal virome of these bats, as viruses excreted in fecal material or which may be replicating in rectal or intestinal tissues have the greatest opportunities of coming into contact with other hosts. Samples were collected in five regions of South Africa over eight years. Initial virome composition was determined by viral metagenomic sequencing by pooling samples and enriching for viral particles. Libraries were sequenced on the Illumina MiSeq and NextSeq500 platforms, producing a combined 37 million reads. Bioinformatics analysis of the high throughput sequencing data detected the full genome of a novel species of the Circoviridae family, and also identified sequence data from the Adenoviridae, Coronaviridae, Herpesviridae, Parvoviridae, Papillomaviridae, Phenuiviridae, and Picornaviridae families. Metagenomic sequencing data was insufficient to determine the viral diversity of certain families due to the fragmented coverage of genomes and lack of suitable sequencing depth, as some viruses were detected from the analysis of reads-data only. Follow up conventional PCR assays targeting conserved gene regions for the Adenoviridae, Coronaviridae, and Herpesviridae families were used to confirm metagenomic data and generate additional sequences to determine genetic diversity. The complete coding genome of a MERS-related coronavirus was recovered with additional amplicon sequencing on the MiSeq platform. The new genome shared 97.2% overall nucleotide identity to a previous Neoromicia-associated MERS-related virus, also from South Africa. Conventional PCR analysis detected diverse adenovirus and herpesvirus sequences that were widespread throughout Neoromicia populations in South Africa. Furthermore, similar adenovirus sequences were detected within these populations throughout several years. With the exception of the coronaviruses, the study represents the first report of sequence data from several viral families within a Southern African insectivorous bat genus; highlighting the need for continued investigations in this regard.Item Self-adjoint higher order differential operators with eigenvalue parameter dependent boundary conditions.(SpringerOpen [Commercial Publisher], 2015-12) Möller, Manfred; Zinsou, BertinEigenvalue problems for even order regular quasi-differential equations with boundary conditions which depend linearly on the eigenvalue parameter λ can be represented by an operator polynomial (Formula presented.) where M is a self-adjoint operator. Necessary and sufficient conditions are given such that also K and A are self-adjoint.Item Stabilization of ODE with hyperbolic equation actuator subject to boundary control matched disturbance(Taylor and Francis Ltd., 2019-01-02) Zhou, H.C.; Guo, B.Z.In this paper, we consider stabilisation for a cascade of ODE and first-order hyperbolic equation with external disturbance flowing to the control end. The active disturbance rejection control (ADRC) and sliding mode control (SMC) approaches are adopted in investigation. By ADRC approach, the disturbance is estimated through a disturbance estimator with both time-varying high gain and constant high gain, and the disturbance is canceled online in the feedback loop. It is shown that the resulting closed-loop system with time-varying high gain is asymptotically stable and is practically stable with constant high gain. By SMC approach, the existence and uniqueness of the solution for the closed loop via SMC are proved, and the monotonicity of the ‘reaching condition’ is presented. The resulting closed-loop system is shown to be exponentially stable. The numerical experiments are carried out to illustrate effectiveness of the proposed control law. © 2016, © 2016 Informa UK Limited, trading as Taylor & Francis Group.Item Surface moisture and vegetation cover analysis for drought monitoring in the southern Kruger National Park using Sentinel-1, Sentinel-2, and Landsat-8(MDPI, 2018-09) Urban, M.; Berger, C.; Mudau, T.E.; Heckel, K.; Truckenbrodt, J.; Odipo, V.O.; Smit, I.P.J.During the southern summer season of 2015 and 2016, South Africa experienced one of the most severe meteorological droughts since the start of climate recording, due to an exceptionally strong El Niño event. To investigate spatiotemporal dynamics of surface moisture and vegetation structure, data from ESA's Copernicus Sentinel-1/-2 and NASA's Landsat-8 for the period between March 2015 and November 2017were utilized. In combination, these radar and optical satellite systems provide promising data with high spatial and temporal resolution. Sentinel-1 C-band data was exploited to derive surface moisture based on a hyper-temporal co-polarized (vertical-vertical-VV) radar backscatter change detection approach, describing dynamics between dry and wet seasons. Vegetation information from a TLS (Terrestrial Laser Scanner)-derived canopy height model (CHM), as well as the normalized difference vegetation index (NDVI) from Sentinel-2 and Landsat-8, were utilized to analyze vegetation structure types and dynamics with respect to the surface moisture index (SurfMI). Our results indicate that our combined radar-optical approach allows for a separation and retrieval of surface moisture conditions suitable for drought monitoring. Moreover, we conclude that it is crucial for the development of a drought monitoring system for savanna ecosystems to integrate land cover and vegetation information for analyzing surface moisture dynamics derived from Earth observation time series.Item Survival analysis of bank loans in the presence of long-term survivors(South African Statistical Association, 2017-04) Marimo, M; Chimedza, CIn this paper we model competing risks, default and early settlement events, in the presence of long term survivors and compare survival and logistic methodologies. Cause specific Cox regression models were fitted and adjustments were made to accommodate a proportion of long term survivors. Methodologies were compared using ROC curves and area under the curves. The results show that survival methods perform better than logistic regression methods when modelling lifetime data in the presence of competing risks and in the presence of long term survivors.Item Thermal analysis of natural convection and radiation heat transfer in moving porous fins(Global Digital Central, 2019) Ndlovu, P.L.; Moitsheki, R.J.In this article, the Differential Transform Method (DTM) is used to perform thermal analysis of natural convective and radiative heat transfer in moving porous fins of rectangular and exponential profiles. This study is performed using Darcy’s model to formulate the governing heat transfer equations. The effects of porosity parameter, irregular profile and other thermo-physical parameters, such as Peclet number and the radiation parameter are also analyzed. The results show that the fin rapidly dissipates heat to the surrounding temperature with an increase in the values of the porosity parameter and the dimensionless time parameter. The results also show that the heat transfer rate in an exponential profile with negative power factor is much higher than the rectangular profile.Item Transplant Experiments Point to Fire Regime as Limiting Savanna Tree Distribution(Frontiers Media, 2018-09-18) Stevens, N.; Archibald, S.; Bond, W.J.Plant species range shifts are predicted to occur in response to climate change. The predictions are often based on the assumption that climate is the primary factor limiting the distribution of species. However the distribution of grassy biomes in Africa cannot be predicted by climate alone, instead interactions between vegetation, climate and disturbance structure the ecosystems. To test if climatic variables, as predicted by an environmental niche model, determine the distribution limits of two common savanna tree species we established a transplant experiment at a range of latitudes and altitudes much broader than the distribution limits of our study species. We planted seedlings of two common savanna trees, Senegalia nigrescens and Colophospermum mopane, at eight paired high and low elevation sites across an 850 km latitudinal gradient in South African savannas. At each site seedlings were planted in both grassy and cleared plots. After 2 years of growth, rainfall, temperature and location inside or outside their distribution range did not explain species success. Grass competition was the only variable that significantly affected plant growth rates across all sites, but grass competition alone could not explain the distribution limit. Species distributions were best predicted when maximum tree growth rates were considered in relation to local fire return intervals. The probability of sapling escape from the fire trap was the most likely determinant of distribution limits of these two species. As trees grew and survived 100 s of kilometers south of their current range limits we conclude that climate alone does not explain the current distribution of these trees, and that climate change adaptation strategies for savanna environments based only on climatic envelope modeling will be inappropriate.Item Wishart exponential families on cones related to tridiagonal matrices(Springer Tokyo, 2019-04-01) Graczyk, P; Ishi, H; Mamane, SLet G be the graph corresponding to the graphical model of nearest neighbor interaction in a Gaussian character. We study Natural Exponential Families (NEF) of Wishart distributions on convex cones Q G and P G , where P G is the cone of tridiagonal positive definite real symmetric matrices, and Q G is the dual cone of P G . The Wishart NEF that we construct include Wishart distributions considered earlier for models based on decomposable(chordal) graphs. Our approach is, however, different and allows us to study the basic objects of Wishart NEF on the cones Q G and P G . We determine Riesz measures generating Wishart exponential families on Q G and P G , and we give the quadratic construction of these Riesz measures and exponential families. The mean, inverse-mean, covariance and variance functions, as well as moments of higher order, are studied and their explicit formulas are given. © 2018, The Institute of Statistical Mathematics, Tokyo.