Plaque quantitiation through protein measurement

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dc.contributor.authorSmit, A. M.
dc.contributor.authorCleaton-Jones, P. E.
dc.contributor.authorBoardman, M. E.
dc.date.accessioned2016-04-29T12:01:40Z
dc.date.available2016-04-29T12:01:40Z
dc.date.issued1992
dc.descriptionPKen_ZA
dc.description.abstractThis study was undertaken to establish whether the quantitation of dental plaque protein by a dye-binding method (Coomassie G-250) may be used as an index of the amount of dental plaque sampled. Ten sites were sampled in 34 children on 5 occasions at 4 month intervals. The mean protein concentration in 1391 plaque samples was 6.9 +/- 4.1 micrograms (micrograms) (mean +/- standard deviation). A three-way analysis of variance showed that the plaque protein concentration was similar at the different sampling sites in the same child (p = 0.14), but statistically significant differences were observed with respect to time of sampling (F = 36.24; p = 0.0001) and individual sampled (F = 5.69; p = 0.0001). These observations indicate that plaque bacterial counts may be expressed as units of protein concentration and this method may be useful to relate the number of viable bacteria to an estimate of the amount of plaque collected. This ratio allows standardisation for any variation in the amount of plaque collected.en_ZA
dc.identifier.citationSmit, A. M., Cleaton-Jones, P. E., Boardman, M. E. 1992. Plaque quantitiation through protein measurement. JOurnal of the dental association of South Africa; 47(7):333-6en_ZA
dc.identifier.urihttp://hdl.handle.net/10539/20294
dc.language.isoenen_ZA
dc.subjectBacterial Proteins/analysisen_ZA
dc.subjectDental Plaque/chemistryen_ZA
dc.subjectDental Plaque/microbiologyen_ZA
dc.titlePlaque quantitiation through protein measurementen_ZA
dc.typeArticleen_ZA
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