Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells.

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dc.citation.doi10.1371/journal.pone.0096268en_ZA
dc.citation.issue5en_ZA
dc.contributor.authorKhumalo, T.
dc.contributor.authorReusch, U.
dc.contributor.authorKnackmuss, S.
dc.contributor.authorLittle, M.
dc.contributor.authorWeiss, S.F.T.
dc.contributor.authorChetty, C.
dc.contributor.authorDa Costa Dias, B.
dc.date.accessioned2016-10-17T10:03:37Z
dc.date.available2016-10-17T10:03:37Z
dc.date.issued2014-05-05
dc.description.abstractTwo key events, namely adhesion and invasion, are pivotal to the occurrence of metastasis. Importantly, the 37 kDa/67 kDa laminin receptor (LRP/LR) has been implicated in enhancing these two events thus facilitating cancer progression. In the current study, the role of LRP/LR in the adhesion and invasion of liver cancer (HUH-7) and leukaemia (K562) cells was investigated. Flow cytometry revealed that the HUH-7 cells displayed significantly higher cell surface LRP/LR levels compared to the poorly-invasive breast cancer (MCF-7) control cells, whilst the K562 cells displayed significantly lower cell surface LRP/LR levels in comparison to the MCF-7 control cells. However, Western blotting and densitometric analysis revealed that all three tumorigenic cell lines did not differ significantly with regards to total LRP/LR levels. Furthermore, treatment of liver cancer cells with anti-LRP/LR specific antibody IgG1-iS18 (0.2 mg/ml) significantly reduced the adhesive potential of cells to laminin-1 and the invasive potential of cells through the ECM-like Matrigel, whilst leukaemia cells showed no significant differences in both instances. Additionally, Pearson's correlation coefficients suggested direct proportionality between cell surface LRP/LR levels and the adhesive and invasive potential of liver cancer and leukaemia cells. These findings suggest the potential use of anti-LRP/LR specific antibody IgG1-iS18 as an alternative therapeutic tool for metastatic liver cancer through impediment of the LRP/LR- laminin-1 interaction.en_ZA
dc.description.librarianNCS2016en_ZA
dc.description.sponsorshipNational Research Foundation.en_ZA
dc.identifier.citationChetty, C. et al.2014. Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells. PLOS ONE 9 (5):e96268.en_ZA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10539/21211
dc.journal.titlePLoS ONEen_ZA
dc.journal.volume9en_ZA
dc.language.isoenen_ZA
dc.publisherPublic Library of Science.en_ZA
dc.subjectIgG1 iS18en_ZA
dc.subjectimmunoglobulin Gen_ZA
dc.subjectmatrigelen_ZA
dc.subjectreceptor antibodyen_ZA
dc.subjectunclassified drugen_ZA
dc.subjectantiidiotypic antibodyen_ZA
dc.subjectimmunoglobulin Gen_ZA
dc.subjectlamininen_ZA
dc.subjectlaminin 1en_ZA
dc.subjectlaminin receptoren_ZA
dc.subjectantibody specificityen_ZA
dc.subjectbreast canceren_ZA
dc.subjectcancer inhibitionen_ZA
dc.subjectcell adhesionen_ZA
dc.subjectcell levelen_ZA
dc.subjectcell surfaceen_ZA
dc.subjectcontrolled studyen_ZA
dc.subjectdensitometryen_ZA
dc.subjectleukemiaen_ZA
dc.titleAnti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells.en_ZA
dc.typeArticleen_ZA
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