Utilisation of antibody technology to purify CMV-induced plasma membrane proteins
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Date
2015
Authors
Roig, Pilar Farran
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Abstract
Several human cytomegalovirus (HCMV) induced proteins have been found in
human embryonic lung fibroblast cells (HEF). Monospecific antibodies
against three of the glycoproteins (54Kd, 62Kd and 90Kd) raised in rabbits
were used to purify the different glycoproteins from HEF membrane preparations
through affinity chromatography columns. The optimal conditions
for the membrane preparation and the affinity chromatography columns were
determined.