Evaluation and Design of Affordable and Novel HIV-1 Drug Resistance Assays:

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dc.contributor.authorWallis, Carole Lorraine
dc.date.accessioned2006-11-17T11:41:08Z
dc.date.available2006-11-17T11:41:08Z
dc.date.issued2006-11-17T11:41:08Z
dc.descriptionFaculty of Health Sciences, Master of Science in Medicine, 9803855een
dc.description.abstractApproximately 5 million individuals are infected with HIV/AIDS in South Africa. The South African government has initiated a National Anti-retroviral therapy (ARV) Program to manage this disease. The emergence of drug resistance to ARV therapy is of great concern. Commercial gold standard sequence-based genotyping assays for monitoring resistance are unaffordable. This project aimed at developing affordable methods to detect specific point mutations relevant to HIV-1 subtype C. The Oligonucleotide Ligation assay (OLA), a real-time PCR assay and a Restriction Fragment Length Polymorphism (RFLP) assay were explored. Results were compared to the Viroseq genotyping assay. OLA performed poorly on HIV-1 subtype C samples and needs modification. The real-time PCR assay using short Minor Groove Binding probes, accurately detected the K65R mutation. The Mae III RFLP assay detected all V106M mutations accurately. Longitudinal cohort studies are required to confirm relevant mutations, appropriate assays and algorithms for resistance monitoring in HIV-1 subtype C.en
dc.format.extent5269853 bytes
dc.format.extent62793 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10539/1865
dc.language.isoenen
dc.subjectHIVen
dc.subjectPCRen
dc.subjectARVen
dc.subjectSouth Africaen
dc.subjectdiseaseen
dc.titleEvaluation and Design of Affordable and Novel HIV-1 Drug Resistance Assays:en
dc.typeThesisen
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