Characterisation of non-polio enteroviruses identified in disease biomes in South Africa
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Date
2016
Authors
Howard, Wayne
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Abstract
Human enteroviruses (family Picornaviridae) consist of 106 serotypes and are divided
into four species: Human enterovirus (HEV)–A, B, C, and D. Enteroviruses cause a
variety of clinical symptoms from severe (e.g. acute flaccid paralysis) to less severe
(e.g. hand-foot-and-mouth disease). Whilst there is currently no antiviral treatment,
viral genotyping allows for: identification of increased virulence, identification of new
enteroviruses, correlation of virus types with immunity, epidemiological investigations
and provides information on viral inter-relationships. A comprehensive study is
underway to determine the prevalence and type of circulating non-polio enteroviruses
in South Africa, specifically for those involved in recent outbreaks. This study
investigated the prevalence of non-polio enteroviruses circulating in South African
between 2010 and 2012 using samples obtained from 2 national surveillance
programs conducted at the National Institute for Communicable Diseases: Acute
Flaccid Paralysis (AFP) and Rotavirus. Typing was performed using a Real-Time
PCR (RT-PCR) assay, followed by Sanger sequencing. 832 samples were tested to
date (562 from the Rotavirus and 270 from the AFP surveillance programs,
respectively). 446 positive enterovirus samples were detected from which 308
samples were successfully sequenced. Specimens from the AFP program yielded
mostly HEV-B serotypes (90.40%), whereas samples typed directly from the
Rotavirus program stools yielded mostly HEV-C serotypes (47.20%). 92.8% of typed
samples were from patients under 5 years. Despite most detections being HEV-B
(56.55%), the most commonly detected virus was Enterovirus 99 (8.63%) from the
HEV-C species. RT-PCR and sequencing, whilst more expensive, have proven more
accurate than cell culture and neutralization assays for typing enteroviruses. In South
Africa, HEV-B viruses were predominant, and in comparison to studies from other
countries, a larger proportion of HEV-C viruses were detected. Detecting EV directly
from stool yielded a larger diversity of the viruses, and while disease associated
viruses were detected, they did not contribute significantly to the associated disease
burden.
Description
A thesis submitted to the Faculty of Health Sciences, University of Witwatersrand, in
fulfilment of the requirements for the degree of Master of Science in Medicine in
Virology
Johannesburg, 2016