The agrocin encoding function of agrobacterium tumefaciens
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Date
2015-08-03
Authors
Hendson, Mavis
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Abstract
Agrobacterium tumefaciens causes crown gall disease in
dicotyledonous plants, reducing yield and therefore
is a major economic problem. Biological control of this
disease is desirable; other methods arc costly or
impractical. An agrocin productivity
K84, is used to control crown gall. However, many
South African strains are resistant to K84. One aim
of this study was to isolate new agrocin producing strains
with a broader host spectrum. Another was to transfer
the agrocin 84 encoding plasmid to a benign soil species,
Rhizobim. One strain isolated, D286 (biotype 1), produced
a broad host range agrocin active against strains carrying nepaline, octopine or agropineTi plasmids. Agrocin was produced briefly in log phase. Strain D286 harboured a large cryptic plasmid and a smaller plasmid which may encode agrocin production...
Description
A thesis sukmltted to the Faculty af Science, hiversity
the Witrwatersrand, Johannesburg, foi the Degree of Doctor
of Philosophy.
Johannesburg, 1983.