Gene flowanalysis of anopheles arabiensis (Diptera:culicidae) populations in southern africa using microsatellite DNA markers

Simple item page
dc.contributor.authorMouatcho, Joel Claude
dc.date.accessioned2006-10-26T13:28:13Z
dc.date.available2006-10-26T13:28:13Z
dc.date.issued2006-10-26T13:28:13Z
dc.description0009014A Msc thesis Science Animal plant and environmental sciencesen
dc.description.abstractAnopheles arabiensis is considered an important vector of human malaria in the southern African region where the disease is the major cause of morbidity and mortality. Gene flow plays an important role in malaria control with the spread of insecticide resistance. The main objectives of this study were to (i) measure the genetic variability within and between five populations (Botswana, Namibia, South Africa, Swaziland and Zimbabwe) of wild An. arabiensis and (ii) estimate the level of gene flow between natural populations across the Southern-limits of An. arabiensis. A total of 1225 An. arabiensis specimens were identified out of 1300 mosquitoes collected from 2000-2003 with the sample sizes ranging from 180-292 per country. Variation at four microsatellite markers was investigated on non-denaturing polyacrylamide gels. The results showed fewer variations between populations (2.96%) than within populations (82.60%) suggesting considerable homogeneity. However, estimates of gene flow (Nm) calculated from mean FST and RST statistics were relatively low, 1.14 and 1.19 respectively, suggesting somewhat restricted gene flow between populations. The occurrence of gene flow within subpopulations of An. arabiensis in Zimbabwe but not in South Africa is interesting with regard to the spread of insecticide resistance in Zimbabwe. The results presented here are obviously subject to the limitations inherent in manual, silver staining method of analysing microsatellite DNA markers. It is possible that a different set of results would be obtained if an Automated Sequencing Analyzer were used. iien
dc.format.extent1404583 bytes
dc.format.extent18484 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10539/1477
dc.language.isoenen
dc.subjectanopheles arabiensisen
dc.subjectmicrosatellite DNA markersen
dc.subjectgene flowen
dc.subjectsouthern africaen
dc.titleGene flowanalysis of anopheles arabiensis (Diptera:culicidae) populations in southern africa using microsatellite DNA markersen
dc.typeThesisen
Files
Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Mouatcho J - MSc Abstract.pdf
Size:
18.05 KB
Format:
Adobe Portable Document Format
Description:
Title and abstract
Download
No Thumbnail Available
Name:
Mouatcho J - MSc dissertation.pdf
Size:
1.34 MB
Format:
Adobe Portable Document Format
Description:
Table of contents-reference
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
95 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
ETD Collection