Knock-down of the 37kDa/67kDa laminin receptor LRP/LR impedes telomerase activity.

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dc.citation.doi10.1371/journal.pone.0141618.en_ZA
dc.citation.issue11en_ZA
dc.contributor.authorNaidoo, K.
dc.contributor.authorMalindisa, S.T.
dc.contributor.authorOtgaar, T.C.
dc.contributor.authorDa Costa Dias, B.
dc.contributor.authorFerreirra, E.
dc.contributor.authorReusch, U.
dc.contributor.authorKnackmuss, S.
dc.contributor.authorLittle, M.
dc.contributor.authorWeiss, S.F.T.
dc.contributor.authorLetsolo, B.T.
dc.date.accessioned2016-09-16T10:02:02Z
dc.date.available2016-09-16T10:02:02Z
dc.date.issued2015-11-06
dc.description.abstractCancer has become a major problem worldwide due to its increasing incidence and mortality rates. Both the 37kDa/67kDa laminin receptor (LRP/LR) and telomerase are overexpressed in cancer cells. LRP/LR enhances the invasiveness of cancer cells thereby promoting metastasis, supporting angiogenesis and hampering apoptosis. An essential component of telomerase, hTERT is overexpressed in 85-90% of most cancers. hTERT expression and increased telomerase activity are associated with tumor progression. As LRP/LR and hTERT both play a role in cancer progression, we investigated a possible correlation between LRP/LR and telomerase. LRP/LR and hTERT co-localized in the perinuclear compartment of tumorigenic breast cancer (MDA-MB231) cells and non-tumorigenic human embryonic kidney (HEK293) cells. FLAGĀ® Co-immunoprecipitation assays confirmed an interaction between LRP/LR and hTERT. In addition, flow cytometry revealed that both cell lines displayed high cell surface and intracellular LRP/LR and hTERT levels. Knock-down of LRP/LR by RNAi technology significantly reduced telomerase activity. These results suggest for the first time a novel function of LRP/LR in contributing to telomerase activity. siRNAs targeting LRP/LR may act as a potential alternative therapeutic tool for cancer treatment by (i) blocking metastasis (ii) promoting angiogenesis (iii) inducing apoptosis and (iv) impeding telomerase activity.en_ZA
dc.description.librarianNCS2016en_ZA
dc.description.sponsorshipThis work was supported by the National Research Foundation, the Republic of South Africa.en_ZA
dc.identifier.citationNaidoo, K. et al. 2015. Knock-down of the 37kDa/67kDa laminin receptor LRP/LR impedes telomerase activity. PLoS ONE 10(11): e0141618.en_ZA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10539/21037
dc.journal.titlePLOS ONEen_ZA
dc.journal.volume10en_ZA
dc.language.isoenen_ZA
dc.publisherPublic Library of Science.en_ZA
dc.rightsĀ© 2015 Naidoo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.subjectlaminin receptor;en_ZA
dc.subjectsmall interfering RNA;en_ZA
dc.subjecttelomerase;en_ZA
dc.subjectbreast cancer;en_ZA
dc.subjectcancer cell line;en_ZA
dc.subjectcell level;en_ZA
dc.subjectcell nucleus;en_ZA
dc.subjectcell surface;en_ZA
dc.subjectcontrolled study;en_ZA
dc.subjectembryo;en_ZA
dc.subjectenzyme activity;en_ZA
dc.subjectgene silencing;en_ZA
dc.subjectHEK293 cell line;en_ZA
dc.subjecthuman; human cell;en_ZA
dc.subjectMB231 cell line;en_ZA
dc.subjectprotein function;en_ZA
dc.subjectprotein localization;en_ZA
dc.subjectprotein protein interaction;en_ZA
dc.subjectRNA interference;en_ZA
dc.titleKnock-down of the 37kDa/67kDa laminin receptor LRP/LR impedes telomerase activity.en_ZA
dc.typeArticleen_ZA
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