In Vitro Inhibition of Angiogenesis by Antibodies Directed against the 37kDa/67kDa Laminin Receptor

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dc.citation.doi10.1371/journal.pone.0058888en_ZA
dc.citation.issue3en_ZA
dc.contributor.authorKhusal, R.
dc.contributor.authorDa Costa Dias, B
dc.contributor.authorMoodley, K.
dc.contributor.authorPenny, C.
dc.contributor.authorReusch, U.
dc.contributor.authorKnackmuss, S.
dc.contributor.authorLittle, M.
dc.contributor.authorWeiss, S.F.T.
dc.date.accessioned2016-10-25T12:58:59Z
dc.date.available2016-10-25T12:58:59Z
dc.date.issued2013-03-12
dc.description.abstractThe 37kDa/67kDa laminin receptor (LRP/LR) is a central receptor mediating interactions between tumour cells and the basement membrane and is thereby a key player in adhesion and invasion, essential processes in metastatic cancer. To affect continued tumour growth, tumours induce angiogenesis for the constant delivery of nutrients and oxygen. This study aims to determine the blocking effect of the anti-LRP/LR specific antibody, W3 on the angiogenic potential of HUVE (human umbilical vein endothelial) cells. Flow cytometric analysis revealed that 97% of HUVE cells display cell surface LRP/LR. An angiogenesis assay was conducted employing HUVE cells seeded on the basement membrane reconstituent Matrigel™ supplemented with the pro-angiogenic factor vascular endothelial growth factor (VEGF). Post 18h incubation at 37°C tubular structures, namely tube lengths were assessed. Treatment of established tubular structures with 100 μg/ml anti-LRP/LR specific antibody completely blocked angiogenesis. Our findings suggest a central role of the 37kDa/67kDa LRP/LR in tube formation and recommends anti-LRP/LR specific antibodies as potential therapeutic tools for treatment of tumour angiogenesis.en_ZA
dc.description.librarianNCS2016en_ZA
dc.description.sponsorshipThis work was supported by the National Research Foundation, the Republic of South Africa and the Medical Research Council, the Republic of South Africa.en_ZA
dc.identifier.citationKhusal, R. et al. 2013. In Vitro Inhibition of Angiogenesis by Antibodies Directed against the 37kDa/67kDa Laminin Receptor. PLoS ONE 8(3):e58888en_ZA
dc.identifier.issn1932-6203.
dc.identifier.urihttp://hdl.handle.net/10539/21272
dc.journal.titlePLoS ONEen_ZA
dc.journal.volume8en_ZA
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights© 2013 Khusal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.subjectlaminin receptoren_ZA
dc.subjectmatrigelen_ZA
dc.subjectpolyclonal antibodyen_ZA
dc.subjectunclassified drugen_ZA
dc.subjectvasculotropinen_ZA
dc.subjectW3 antibodyen_ZA
dc.subjectantiangiogenic activityen_ZA
dc.subjectantibody specificityen_ZA
dc.subjectbasement membraneen_ZA
dc.subjectcancer cellen_ZA
dc.subjectAngiogenesis Inhibitorsen_ZA
dc.subjectAntibodiesen_ZA
dc.subjectMonoclonalen_ZA
dc.subjectAntigensen_ZA
dc.subjectCD31en_ZA
dc.subjectCell Membraneen_ZA
dc.subjectCells, Cultureden_ZA
dc.subjectHuman Umbilical Vein Endothelial Cellsen_ZA
dc.subjectHumansen_ZA
dc.subjectNeovascularization, Physiologicen_ZA
dc.subjectReceptors, Lamininen_ZA
dc.subjectVascular Endothelial Growth Factor Aen_ZA
dc.titleIn Vitro Inhibition of Angiogenesis by Antibodies Directed against the 37kDa/67kDa Laminin Receptoren_ZA
dc.typeArticleen_ZA
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