Assessing Global Transcriptome Changes in Response to South African Cassava Mosaic Virus [ZA-99] Infection in Susceptible Arabidopsis thaliana.

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dc.citation.doi10.1371/journal.pone.0067534.en_ZA
dc.citation.issue6en_ZA
dc.contributor.authorPierce, E.J.
dc.contributor.authorRey, M.E.C.
dc.date.accessioned2016-10-17T10:39:37Z
dc.date.available2016-10-17T10:39:37Z
dc.date.issued2013-06-27
dc.description.abstractIn susceptible plant hosts, co-evolution has favoured viral strategies to evade host defenses and utilize resources to their own benefit. The degree of manipulation of host gene expression is dependent on host-virus specificity and certain abiotic factors. In order to gain insight into global transcriptome changes for a geminivirus pathosystem, South African cassava mosaic virus [ZA:99] and Arabidopsis thaliana, 4×44K Agilent microarrays were adopted. After normalization, a log2 fold change filtering of data (p<0.05) identified 1,743 differentially expressed genes in apical leaf tissue. A significant increase in differential gene expression over time correlated with an increase in SACMV accumulation, as virus copies were 5-fold higher at 24 dpi and 6-fold higher at 36 dpi than at 14 dpi. Many altered transcripts were primarily involved in stress and defense responses, phytohormone signalling pathways, cellular transport, cell-cycle regulation, transcription, oxidation-reduction, and other metabolic processes. Only forty-one genes (2.3%) were shown to be continuously expressed across the infection period, indicating that the majority of genes were transient and unique to a particular time point during infection. A significant number of pathogen-responsive genes were suppressed during the late stages of pathogenesis, while during active systemic infection (14 to 24 dpi), there was an increase in up-regulated genes in several GO functional categories. An adaptive response was initiated to divert energy from growth-related processes to defense, leading to disruption of normal biological host processes. Similarities in cell-cycle regulation correlated between SACMV and Cabbage leaf curl virus (CaLCuV), but differences were also evident. Differences in gene expression between the two geminiviruses clearly demonstrated that, while some global transcriptome responses are generally common in plant virus infections, temporal host-specific interactions are required for successful geminivirus infection. To our knowledge this is the first geminivirus microarray study identifying global differentially expressed transcripts at 3 time points.en_ZA
dc.description.librarianNCS2016.en_ZA
dc.description.sponsorshipFunding was from the National Bioinformatics Network through the Department of Science and Technology (www.dst.gov.za) and the National Research Foundation (www.nrf.ac.za).en_ZA
dc.identifier.citationPierce, E.J. and Rey, M.E.C.en_ZA
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10539/21214
dc.journal.titlePLoS ONE.en_ZA
dc.journal.volume8en_ZA
dc.language.isoenen_ZA
dc.publisherPublic Library of Science.en_ZA
dc.rights© 2013 Pierce, Rey. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.subjectphytohormoneen_ZA
dc.subjectplant RNAen_ZA
dc.subjecttranscriptomeen_ZA
dc.subjectArabidopsis thalianaen_ZA
dc.subjectcell cycle regulationen_ZA
dc.subjectcell transporten_ZA
dc.subjectcoevolutionen_ZA
dc.subjectevolutionary adaptationen_ZA
dc.subjectGeminivirusen_ZA
dc.subjectgene expressionen_ZA
dc.subjectgene repressionen_ZA
dc.subjectgenetic transcriptionen_ZA
dc.subjecthost pathogen interactionen_ZA
dc.subjecthost rangeen_ZA
dc.subjectimmune evasionen_ZA
dc.subjectmicroarray analysisen_ZA
dc.subjectoxidation reduction reactionen_ZA
dc.subjectsignal transductionen_ZA
dc.subjectSouth African cassava mosaic virusen_ZA
dc.subjectSouth African cassava mosaic virus infectionen_ZA
dc.subjectviral plant diseaseen_ZA
dc.subjectvirus loaden_ZA
dc.subjectvirus pathogenesisen_ZA
dc.titleAssessing Global Transcriptome Changes in Response to South African Cassava Mosaic Virus [ZA-99] Infection in Susceptible Arabidopsis thaliana.en_ZA
dc.typeArticleen_ZA
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