National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012
dc.contributor.author | Perovic, O. | |
dc.contributor.author | Singh- Moodley, A. | |
dc.contributor.author | Duse, A. | |
dc.contributor.author | Elliott, G. | |
dc.contributor.author | Bamford, C. | |
dc.contributor.author | Swe Swe-Han, K. | |
dc.contributor.author | R Kularatne, R. | |
dc.contributor.author | Lowman, W. | |
dc.contributor.author | Whitelaw, A. | |
dc.contributor.author | Nana, T. | |
dc.contributor.author | Wadula, J. | |
dc.contributor.author | Lekalakala, R. | |
dc.contributor.author | Saif, A. | |
dc.contributor.author | Marais, E. | |
dc.date.accessioned | 2015-06-10T12:53:24Z | |
dc.date.available | 2015-06-10T12:53:24Z | |
dc.date.issued | 2014-08 | |
dc.department | Clinical Microbiology and Infectious Diseases | |
dc.description.abstract | Background: The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are of major public health concern worldwide. Objectives: To describe the antibiotic susceptibility profiles of K. pneumoniae isolates from bacteraemic patients submitted by sentinel laboratories in five regions of South Africa from mid-2010 to mid-2012. Molecular methods were used to detect the most commonly found extended-spectrum beta lactamase (ESBL) and carbapenemase resistance genes. Methods: Thirteen academic centres serving the public healthcare sector in Gauteng, KwaZulu-Natal, Free State, Limpopo and Western Cape provinces submitted K. pneumoniae isolates from patients with bloodstream infections. Vitek 2 and MicroScan instruments were used for organism identification and susceptibility testing. Multiplex polymerase chain reactions (PCRs) were used to detect blaCTX-M, blaSHV and blaTEM genes in a proportion of the ESBL isolates. All isolates exhibiting reduced susceptibility to carbapenems were PCR tested for blaKPC and blaNDM-1 resistance genes. Results: Overall, 68.3% of the 2 774 isolates were ESBL-positive, showing resistance to cefotaxime, ceftazidime and cefepime. Furthermore, 46.5% of all isolates were resistant to ciprofloxacin and 33.1% to piperacillin-tazobactam. The major ESBL genes were abundantly present in the sample analysed. Most isolates (95.5%) were susceptible to the carbapenems tested, and no isolates were positive for blaKPC or blaNDM-1. There was a trend towards a decrease in susceptibility to most antibiotics. Conclusion: The high proportion of ESBL-producing K. pneumoniae isolates observed, and the prevalence of ESBL genes, are of great concern. Our findings represent a baseline for further surveillance in SA, and can be used for policy and treatment decisions. | en_ZA |
dc.faculty | Faculty of Health Sciences | |
dc.identifier.citation | Perovic, O., Singh- Moodley, A., Duse, A., et al. 2014. National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012. SAMJ;104(8):563-568. | en_ZA |
dc.identifier.doi | 10.7196/SAMJ.7617 | |
dc.identifier.issn | 0256-9574 (print) | |
dc.identifier.issn | 2078-5135 (online) | |
dc.identifier.uri | http://hdl.handle.net/10539/17970 | |
dc.journal.title | South African Medical Journal (SAMJ) | |
dc.language.iso | en | en_ZA |
dc.publisher | South African Medical Association | |
dc.rights | © The Authors 2014. This article is licensed under a Creative Commons Attribution - Noncommercial Works License. | |
dc.school | School of Pathology | |
dc.subject | Drug Resistance | en_ZA |
dc.subject | Klebsiella pneumoniae | en_ZA |
dc.subject | South Africa | en_ZA |
dc.title | National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012 | en_ZA |
dc.type | Article | en_ZA |
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