National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012

Simple item page
dc.contributor.authorPerovic, O.
dc.contributor.authorSingh- Moodley, A.
dc.contributor.authorDuse, A.
dc.contributor.authorElliott, G.
dc.contributor.authorBamford, C.
dc.contributor.authorSwe Swe-Han, K.
dc.contributor.authorR Kularatne, R.
dc.contributor.authorLowman, W.
dc.contributor.authorWhitelaw, A.
dc.contributor.authorNana, T.
dc.contributor.authorWadula, J.
dc.contributor.authorLekalakala, R.
dc.contributor.authorSaif, A.
dc.contributor.authorMarais, E.
dc.date.accessioned2015-06-10T12:53:24Z
dc.date.available2015-06-10T12:53:24Z
dc.date.issued2014-08
dc.departmentClinical Microbiology and Infectious Diseases
dc.description.abstractBackground: The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are of major public health concern worldwide. Objectives: To describe the antibiotic susceptibility profiles of K. pneumoniae isolates from bacteraemic patients submitted by sentinel laboratories in five regions of South Africa from mid-2010 to mid-2012. Molecular methods were used to detect the most commonly found extended-spectrum beta lactamase (ESBL) and carbapenemase resistance genes. Methods: Thirteen academic centres serving the public healthcare sector in Gauteng, KwaZulu-Natal, Free State, Limpopo and Western Cape provinces submitted K. pneumoniae isolates from patients with bloodstream infections. Vitek 2 and MicroScan instruments were used for organism identification and susceptibility testing. Multiplex polymerase chain reactions (PCRs) were used to detect blaCTX-M, blaSHV and blaTEM genes in a proportion of the ESBL isolates. All isolates exhibiting reduced susceptibility to carbapenems were PCR tested for blaKPC and blaNDM-1 resistance genes. Results: Overall, 68.3% of the 2 774 isolates were ESBL-positive, showing resistance to cefotaxime, ceftazidime and cefepime. Furthermore, 46.5% of all isolates were resistant to ciprofloxacin and 33.1% to piperacillin-tazobactam. The major ESBL genes were abundantly present in the sample analysed. Most isolates (95.5%) were susceptible to the carbapenems tested, and no isolates were positive for blaKPC or blaNDM-1. There was a trend towards a decrease in susceptibility to most antibiotics. Conclusion: The high proportion of ESBL-producing K. pneumoniae isolates observed, and the prevalence of ESBL genes, are of great concern. Our findings represent a baseline for further surveillance in SA, and can be used for policy and treatment decisions.en_ZA
dc.facultyFaculty of Health Sciences
dc.identifier.citationPerovic, O., Singh- Moodley, A., Duse, A., et al. 2014. National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012. SAMJ;104(8):563-568.en_ZA
dc.identifier.doi10.7196/SAMJ.7617
dc.identifier.issn0256-9574 (print)
dc.identifier.issn2078-5135 (online)
dc.identifier.urihttp://hdl.handle.net/10539/17970
dc.journal.titleSouth African Medical Journal (SAMJ)
dc.language.isoenen_ZA
dc.publisherSouth African Medical Association
dc.rights© The Authors 2014. This article is licensed under a Creative Commons Attribution - Noncommercial Works License.
dc.schoolSchool of Pathology
dc.subjectDrug Resistanceen_ZA
dc.subjectKlebsiella pneumoniaeen_ZA
dc.subjectSouth Africaen_ZA
dc.titleNational sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012en_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae in South Africa 2010 2012.pdf
Size:
607.14 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
*School of Pathology (Research Outputs)
Wits Research Outputs