Expression and characterization of functional HIV-1 subtype C envelope glycoproteins in insect cells.

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dc.contributor.authorNkosi., Praise-God Sibusiso.
dc.date.accessioned2019-03-22T09:11:00Z
dc.date.available2019-03-22T09:11:00Z
dc.date.issued2002-09-20
dc.descriptionA dissertation submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, for the degree of Masters of Science in Medicine (Virology). Johannesburg, 2002en_ZA
dc.description.abstractThe full-length envelope glycoproteins from four South African HIV-1 subtype C isolates exhibiting different phenotypic properties were expressed in insect cells, purified, and their biological characteristics investigated. Isolates 98ZA151Du, 99ZACM9, 98ZA179DU and 99ZASW7 that utilize the CCR5, CCR5/CXCR4, CCR5/CXCR4 and CXCR4 coreceptors for cell entry, respectively were selected for this study. All four full-length env genes were PCR amplified, cloned into a baculovirus expression system and the recombinant envelope glycoproteins were expressed in Spodoptera frugiperda (Sf9) insect cells.en_ZA
dc.description.librarianIT2019en_ZA
dc.identifier.urihttps://hdl.handle.net/10539/26616
dc.language.isoenen_ZA
dc.subject.meshGlycoproteins
dc.subject.meshHIV-1
dc.subject.meshSpodoptera
dc.titleExpression and characterization of functional HIV-1 subtype C envelope glycoproteins in insect cells.en_ZA
dc.typeThesisen_ZA

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