The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning.

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dc.citation.doi10.3390/ijms160510242en_ZA
dc.citation.epage10266en_ZA
dc.citation.issue5en_ZA
dc.citation.spage10242en_ZA
dc.contributor.authorHull, R.
dc.contributor.authorOosthuysen, B.
dc.contributor.authorCajee, U.-F.
dc.contributor.authorMokgohloa, L.
dc.contributor.authorNweke, E.
dc.contributor.authorAntunes, R.J.
dc.contributor.authorCoetzer, T.H.T.
dc.contributor.authorNtwasa, M.
dc.date.accessioned2016-09-28T15:05:35Z
dc.date.available2016-09-28T15:05:35Z
dc.date.issued2015-05
dc.description.abstractThe human retinoblastoma binding protein 6 (RBBP6) is implicated in esophageal, lung, hepatocellular and colon cancers. Furthermore, RBBP6 was identified as a strong marker for colon cancer prognosis and as a predisposing factor in familial myeloproliferative neoplasms. Functionally, the mammalian protein interacts with p53 and enhances the activity of Mdm2, the prototypical negative regulator of p53. However, since RBBP6 (known as PACT in mice) exists in multiple isoforms and pact−/− mice exhibit a more severe phenotype than mdm2−/− mutants, it must possess some Mdm2-independent functions. The function of the invertebrate homologue is poorly understood. This is complicated by the absence of the Mdm2 gene in both Drosophila and Caenorhabditis elegans. We have experimentally identified the promoter region of Snama, the Drosophila homologue, analyzed potential transcription factor binding sites and confirmed the existence of an additional isoform. Using band shift and co-immunoprecipitation assays combined with mass spectrometry, we found evidence that this gene may be regulated by, amongst others, DREF, which regulates hundreds of genes related to cell proliferation. The potential transcription factors for Snama fall into distinct functional groups, including anteroposterior embryonic patterning and nucleic acid metabolism. Significantly, previous work in mice shows that pact−/− induces an anteroposterior phenotype in embryos when rescued by simultaneous deletion of p53. Taken together, these observations indicate the significance of RBBP6 proteins in carcinogenesis and in developmental defects.en_ZA
dc.description.librarianSP2016en_ZA
dc.identifier.citationHull, R. et al. 2015. The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning. International Journal of Molecular Sciences 16(5), pp.10242-10266.en_ZA
dc.identifier.issn1661-6596 (Print)
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10539/21092
dc.journal.titleInternational Journal of Molecular Sciencesen_ZA
dc.journal.volume16en_ZA
dc.language.isoenen_ZA
dc.publisherMDPI AGen_ZA
dc.subjectretinoblastoma binding protein 6en_ZA
dc.subjectRBBP6en_ZA
dc.subjectSNAMAen_ZA
dc.subjectp53en_ZA
dc.subjectRben_ZA
dc.subjectMdm2en_ZA
dc.titleThe Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning.en_ZA
dc.typeArticleen_ZA
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