Bacterial studies on debris taken from the gingival sulcus of man

Coogan, Maeve M
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The gingival sulcus micro-ecology of three categories of people was studied. Samples of debris were removed from the gingival sulcus of ten male Bantu subjects with pockets of 1 - 2 mm, ten male Bantu subjects with pockets of 3 - 6 mm and twenty male Caucasian subjects with pockets of 1 — 2 mm. The samples were placed in cooked meat medium which was incubated at 37°C for thirty days. They were plated out daily from the cooked meat onto a variety of media. Organisms cultured were identified to the genus level. Changes in the pH and amino acids in the medium were studied in ten of the samples taken from the Bantu subjects. Organisms isolated were classified as dominants, associates and incidentals. Dominants were stable, had a high incidence and persisted in the medium. They included the aerobic streptococci, actinomyces,C(-haemol'/tic streptococci, mitis-type streptococci and the enterococci. Associates were not stable or did not survive well and could be divided into five groups. The first group consisted of the veillonella, lactobacilli, -haemolytic streptococci and the sarcina. They were favoured by deep pockets. The second group was favoured by shallow pockets and the environment of the Bantu and consisted of the anaerobic streptococci, staphylococci, bacteroides, neisseria, selenomonas, fusobacteria, diplococci, Clostridia, loptotrichia and polysaccharide-producing streptococci. This group contained a number of pathogens and the significance of this finding is discussed. iv/...The third/.