The relationship between theileria parva parva and t.p. lawrencei as shown by sporozoite antigen and ribosomal RNA gene sequences

Simple item page
dc.contributor.authorCollins, Nicola, Elaine
dc.date.accessioned2017-07-05T09:50:08Z
dc.date.available2017-07-05T09:50:08Z
dc.date.issued1997
dc.descriptionA thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfillment of the requirements for the degree of Doctor of Philosophy.en_ZA
dc.description.abstractThe aim of this thesis was to develop DNA probes to distinguish between the protozoan parasites Theileria parva parva and T. p. lawrencei which cause East Coast fever (ECF) and Corridor disease respectively. ECF was eradicated from South Arrlca in 1954, and today Corridor disease has become the most important form of theileriosis. Although ECF has been eradicated, the vector ticks are still prevalent in South Africa and the cattle population would be highly susceptible to a recurrence of the disease, At present there is no reliable means of distinguishing between T.p. parva and T. p. lawrencei. Sequence differences between T. parva and other Theileria species have previously been found in the small subunit ribosomal RNA (rRNA) gene; probes designed to detect these sequence differences Can be used to distinguish between Theileria species. We therefore decided to search for differences in the rRNA genes of T. p. parva and T.p. lawrencei. To this end, the entire "RNA transcription unit was amplified from a cloned T. p, lawrence; parasite; the unit comprises the small subunit rRNA (SSUrRNA) gene, the internal transcribed spacer (ITS) and the large subunit rRNA (LSUrRNA) gene. The amplification products were cloned and sequenced, and the T.p, lawrencei rRNA sequence was compared to that of T. p, parva, While there was little variation in their SSUrRNA and LSUrRNA gene sequences, there was major sequence variation in the ITS The ITSs from twelve T. parva isolates were amplified, cloned and sequenced, and eleven characterisation oligonucleotide probes were identified. The T. p, parva isolates screened in this study hybridised with a limited subset of the probes, While the T. p. lawrencei isolates, hybridised with many more of the probes, indicating that the T. parva population in cattle is more homogenous than that in buffalo. There thus appears to have been a selection in cattle of a relatively homogenous subpopuiation of T. parva from a much larger, more diverse gene pool in buffalo. Although most T.p. parva isolates (93.5%) were detected by probe TPPI, and most T.p, lawrencei isolates (81.8%) were detected byen_ZA
dc.description.librarianAC2017en_ZA
dc.identifier.urihttp://hdl.handle.net/10539/22930
dc.language.isoenen_ZA
dc.subjectTheileria parva -- South Africa.en_ZA
dc.subjectTheileriosis.en_ZA
dc.titleThe relationship between theileria parva parva and t.p. lawrencei as shown by sporozoite antigen and ribosomal RNA gene sequencesen_ZA
dc.typeThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Collins Nicola Elaine._The relationship between Th.pdf
Size:
4.6 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
ETD Collection