Studies towards the development of Salmonella-specific bacteriophages for sanitation in the food industry
| dc.contributor.author | Hobbs, Angela | |
| dc.date.accessioned | 2008-03-18T08:18:32Z | |
| dc.date.available | 2008-03-18T08:18:32Z | |
| dc.date.issued | 2008-03-18T08:18:32Z | |
| dc.description.abstract | ABSTRACT Bacteriophages have sparked interest as novel ways to control foodborne pathogens. The application of Salmonella-specific phages as antimicrobial agents was tested against relevant Salmonella isolates of poultry origin. Two different Salmonella-specific phages, A and C, were isolated from enriched sewage. They displayed differences in their host-range but exhibited virulent behaviour towards Salmonella enterica serovar Typhimurium ATCC 13311. Toxicity studies were conducted with individual and combined applications of phages A and C, at an MOI of 1, on Salmonella ATCC 13311. Following 3 hour exposure, both applications were equally effective at reducing Salmonella by approximately 1 x 105 CFU/ml. Similar toxicity profiles were observed with both applications, however, a delay occurred with phage A. We propose that phages A and C have similar infective specificities and that during combination competition for the receptor is overcome by phage C. Neither application eliminated Salmonella to undetectable levels. The presence of phage-resistant mutants is a fundamental issue that will hamper the use of phages as alternate antimicrobial agents. | en |
| dc.format.extent | 1548975 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10539/4674 | |
| dc.language.iso | en | en |
| dc.subject | Salmonella | en |
| dc.subject | Bacteriophages | en |
| dc.title | Studies towards the development of Salmonella-specific bacteriophages for sanitation in the food industry | en |
| dc.type | Thesis | en |