Preparation of ribsomal subunits by gel filtration
| dc.contributor.author | Bhoolia, Deena | |
| dc.date.accessioned | 2016-07-20T12:25:15Z | |
| dc.date.available | 2016-07-20T12:25:15Z | |
| dc.date.issued | 2016-07-20 | |
| dc.description | A Oiss{!y'tation $uhm'itted in fulfilment of the r~;~airements, for' the degree of f·1aster· oi~'j Science at the University of the ~titwatersrand ' Juhanoasbul"g January 1991 | en_ZA |
| dc.description.abstract | An attempt was made to separate ribosomal subunits by gel filtl'ation on Trisacryl GF2000 and Sepharose 4B. Trisacryl GP~2000,a sYllthet'ic gol, , separated rat .ljver rlbosonal subunits orl 'f~'135,em column with a resolution of ""Or3, resulting 'in <'1" 60 rJ impm''ity 'of each of the \~ subunits. subunits" were not resolved. Sepharose 4131 an agarose based gol, separated " the subunits by adsorpttcn chromatography rathr.r than 'i>.V () Q ':) At 4°C, the 405 .subunf ts were eluted with a kd () ruO,:~9( but the GOrf' $ubud"its adsorbed to th~.'g,~Jl and were eluted I; /) when the temperature of "the column was increas~d to 250C..3SoC. (' ,. ('-. This edsorpt ion phenonenon seems to b{~ tl propert~ of a 11 agllrose -: \') ~\:; based gels studied here, includ'ing Sepharose 2.B and .seph'ato~eoBt arid is exc 1us iva to !,mamm'i'lan r ibosOIntrt subuni ts • Anal,ys1S 0'(" the , u subunits by in vitro r14C]polypheny1alanine sy'nthes'is showed OCI " ,,'c. /\ I 7', " diff'erence 'in the act lvtt ies of dbosoma'J ~ubunH~ p~epdrf#d by ;/'/ ' grndient centrifugation or by 5epharos,e cni"OmatogNPPY;' Analy~ii ~~of " (~ (( the subunits by ~crylamidec'ugarose coU)po5it~) gs1s resulted ,in the '/ resolution ,.:n"f subunits isolated fr'oill lower organisms in o II non-denaturlnq !Jcl systems and SI,lbut1its from m«mm~nan'tissue in II /'\';1 Ga'! f'iltrat'ion does tyffer a 5t!i:l;~'ble metrKld'¥or the pr~p;n~¥tiol1of <::) . I \) \\ I) ribosoma 1 'subunits I but only' if 'the act~orption JH'OrJ&~"t'Jo,s of ,) (,) ':' o ,1) ,{ | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/10539/20684 | |
| dc.language.iso | en | en_ZA |
| dc.subject.lcsh | Ribosomes | |
| dc.subject.lcsh | Escherichia coli | |
| dc.subject.lcsh | Gel permeation chromatography | |
| dc.subject.lcsh | Liver | |
| dc.title | Preparation of ribsomal subunits by gel filtration | en_ZA |
| dc.type | Thesis | en_ZA |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Bhoolia Deena._Preparation Of Ribosomal Subunits B.pdf
- Size:
- 4.74 MB
- Format:
- Adobe Portable Document Format
- Description:
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: