3. Electronic Theses and Dissertations (ETDs) - All submissions
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Item Activation of JNK1B1 by phosphorylation: implications for its function, stability and dynamics(2015-01-29) Owen, Gavin RayThe c-Jun N-terminal kinases (JNKs) are mitogen-activated protein kinases (MAPKs) that are activated by the dual phosphorylation of a canonical threonine and tyrosine residue. While it is well known that the activation of JNK mediates many important cellular processes such as differentiation, proliferation, and apoptosis, the mechanisms by which phosphorylation induces its activation are not known. An understanding of the structural and biophysical basis for the activation of JNK is highly desirable however, as dysregulation of the kinase has been implicated in numerous prominent diseases. Aiming first to improve upon the previously reported inadequacies in acquiring active JNK, this work describes a novel method for the purification of large yields of pure and phosphorylated JNK1β1, the most abundant JNK isoform. Using codon harmonization as a precautionary measure toward increasing the soluble overexpression of the kinase raised unique questions about the role of translation kinetics in both the heterologous and natural co-translational modification of kinases. After purifying the upstream activating kinases of JNK, phosphorylation of JNK1β1 was achieved by reconstituting the MEKK1 → MKK4 → JNK MAPK activation cascade in vitro. Activated JNK1β1 was thereafter able to phosphorylate its substrate, ATF2, with high catalytic efficiency. Characterising the nature of JNK1β1 modification by MKK4, mass spectrometry revealed that the latter kinase phosphorylates JNK1β1 not only at its activation residues (T183 and Y185), but also at a recognised yet uncharacterised phospho-site (S377) as well as two novel phospho-residues (T228 and S284) whose phosphorylation appear to have functional significance. Unfolding studies and amide hydrogen-deuterium exchange (HX) mass spectrometry (MS) were then used to investigate the changes to the stability and structure/conformational dynamics of JNK1β1 induced by phosphorylation and nucleotide substrate binding. Increased flexibility detected at the hinge between the N- and C-terminal domains upon phosphorylation suggested that activation may require interdomain closure. Patterns of solvent protection by the ATP analogue, AMP-PNP, reflected a novel mode of nucleotide binding to the C-terminal domain of a destabilised and open domain conformation of inactive JNK1β1. HX protection at both domains following AMP-PNP binding to active JNK1β1 revealed that the domains close around nucleotide upon phosphorylation, simultaneously stabilising the kinase. This reveals that phosphorylation activates JNK1β1 in part by enhancing the flexibility of the hinge to enable interdomain closure and the formation of a functional active site. This work thus offers novel insight into the unique molecular mechanisms by which JNK1β1 is regulated by nucleotide binding and phosphorylation by MKK4, and by the complex interplay that exists between them.