School of Oral Health Sciences
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Item Failure at the dental restorative-etched enamel interface(1973) Retief, D. H.Item Failure at the dental restorative etched enamel interface(1973) Retief, D. H.None of the restorative materials available to the dental profession adhere to tooth structure. The bonding of these materials could be greatly increased by etching the enamel surface prior to the placement of the restorative material. Conditioning of the enamel surface with phosphoric acid or an attenuated phosphoric acid solution is now an accepted procedure and widely applied in restorative and preventive dentistry.Item Intercellular junctions in the attached gingiva and alveolar mucosa in the vervet monkey (cercopithecus aethiops)(1979) Grossman, E. S.; Austin, J. C.; Cleaton-Jones, P.This study was undertaken to determine the distribution of hemidesmosomes on the basal cell membranes adjacent to the basement lamina and the spinous cell membranes on unloaded keratinized AG and non- keratinized AM.Item Ultrastructural features of exfoliating surface cells in keratinised and non-keratinised oral mucosa(1979) Austin, J. C.; Grossman, E. S.; Cleaton-Jones, P.Healthy keratinised and non-keratinised oral mucosa (attached gingiva and alveolar mucosa) were obtained from 6 adult vervet monkeys, fixed in situ by simultaneous perfusion and immersion fixation using cacodylate buffered 2,5% glutaraldehyde and prepared for examination by SEM and TEM.Item Sectioning of the inferior dental nerve in rats(1969) Retief, D. H.; Dreyer, C. J.Item Relationship between fluoride concentration in enamel, DMFT Index, and degree of fluorosis in a community residing in an area with a high level of fluoride(1976) Bischoff, J. I.; Van Der Merwe, E. H. M.; Retief, D. H.; et al.The DMFT index, degree of fluorosis (DEGF), fluoride concentration in enamel (F), and depth of etch (D) were determined in participants living in an area with a high fluoride level. A statistically significant difference was found between men and women for F and D. A significant correlation was established between F and DEGF and between F and age for men, but not for women.Item Mitotic activity in the oral epithelium of the albino rat(1976) Cleaton-Jones, P.In groups of Wistar rats 3 and 12 months of age, colchicine and radioautography were used to assess mitotic activity in oral epitheliums. Apparent mitotic activity in the epitheliums of the cheek, hard palate, and central, intermediate, and lateral zones of the soft palate depended on the method used and the age of the rat.Item Radioautographic study of mesenchymal cell activity in the secondary palate of the rat(1976) Cleaton-Jones, P.Fifteen µCi of ³H-thymidine was injected into the amniotic cavity of fetal rats from day 15 to 19 postinsemination. Analysis of radioautographs from these rats did not show increased mesenchymal cell activity to be responsible for medial rotation of the palatal shelves. Variations in cell activity in the older fetuses were mainly due to developing bone and glands.Item A qualitative and quantitative histochemical investigation of the effects of pilocarpine stimulation on amylase levels in rat submaxillary and parotid glands(Sagepub, 1973) Shear, M.; Gibson, S.; van der Merwe, E.A histochemical study, using the starch substrate film technique, was done to compare amylase activity in fresh-frozen cryostat sections of paired male rat parotid and submaxillary-sublingual glands, before and after pilocarpine stimulation. A quantitative procedure was followed on the same material. Amylase depletion was apparent in sections of parotid gland incubated on starch films for short periods. Quantitative estimations on adjacent sections showed an amylase depletion of about 50%. In the submaxillary-sublingual gland, amylase was demonstrable in the blood vessels in both unstimulated and stimulated material and there appeared to be a higher concentration of the enzyme in the latter. Quantitative assays on adjacent sections demonstrated a very considerably raised level of amylase activity in the stimulated submaxillary gland. It was concluded that the histochemically demonstrable amylase in the blood vessels of the submaxillary and sublingual glands was blood amylase and that the raised level following pilocarpine stimulation might be interpreted as a passage to the blood of enzymes produced by other glands under the same stimulus.Item The effect of mechanical loading on the clear cells of the oral epithelium(1979) Fleisch, L.