ETD Collection

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    Lysogeny in encapsulated and nontypable strains of haemophilus influenzae
    (1991) Muthivhi., Tshlidzi
    Limited information on the prevalence of lysogeny and the morphological features of bacteriophages in encapsulated and nontypable strains of Haemophilus influenzae is available from the literature. This study was conducted to investigate lysogeny in wild-type strains of H. influenzae and to characterize bacteriophages recovered from these clinical strains. Following induction by mitomycin C, 16 of 58 isolates of H. influenzae tested produced lysis. Addition of chloramphenicol inhibited cell lysis, indicating that protein synthesis was essential for induction. Type b capsules did not inhibit cell lysis or their ability to become lysoginized
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    Studies towards the development of Salmonella-specific bacteriophages for sanitation in the food industry
    (2008-03-18T08:18:32Z) Hobbs, Angela
    ABSTRACT Bacteriophages have sparked interest as novel ways to control foodborne pathogens. The application of Salmonella-specific phages as antimicrobial agents was tested against relevant Salmonella isolates of poultry origin. Two different Salmonella-specific phages, A and C, were isolated from enriched sewage. They displayed differences in their host-range but exhibited virulent behaviour towards Salmonella enterica serovar Typhimurium ATCC 13311. Toxicity studies were conducted with individual and combined applications of phages A and C, at an MOI of 1, on Salmonella ATCC 13311. Following 3 hour exposure, both applications were equally effective at reducing Salmonella by approximately 1 x 105 CFU/ml. Similar toxicity profiles were observed with both applications, however, a delay occurred with phage A. We propose that phages A and C have similar infective specificities and that during combination competition for the receptor is overcome by phage C. Neither application eliminated Salmonella to undetectable levels. The presence of phage-resistant mutants is a fundamental issue that will hamper the use of phages as alternate antimicrobial agents.