ETD Collection

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Author: search.filters.author.Ferreira, Gracinda Maria NunesSubject: search.filters.subject.Protein kinases

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    Reversible phosphorylation of proteins in proliferating and differentiating cells: cyclic variations and the effect of growth regulators
    (1994) Ferreira, Gracinda Maria Nunes
    Living cells are highly auto-dynamic entities which means that the underlying biochemistry is equally dynamic, a reality which is ignored by most researchers. Theoretical studies indicate that such a state must be due to the existence of oscillatory variations in the levels and activities of key components in the cell. In this study, the dynamic behaviour of four major, interrelated areas of cell biochemistry (phosphorylation, dephosphorylation, the terminal reaction of glycolysis and the amount of soluble protein) were examined and all systems found to oscillate in murine erythroleukaemic cells (MEL) and, where examined, also in the human HL-6Q leukaemic cell line. [Abbreviated Abstract. Open document to view full version]