ETD Collection

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Author: search.filters.author.Chien, Yu, ChenSubject: search.filters.subject.Glutathione transferase

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    Biochemical analysis of the W28F mutant of human class Pi glutathione S-transferase
    (1996) Chien, Yu, Chen
    Glutathione S-transferase (GST) class Pi has two tryptophan residues which are conserved within domain one. Trp38 plays a functional role in sequestering glutathione at the active site, whereas Trp28 plays a structural role. The effects of the sterically-conservative substitution of Trp28 to Phe were investigated. When the W28F mutant was compared with the wild-type enzyme, mutation of Ttp28 to Phe was not well tolerated and resulted in a dimeric protein with impaired catalytic function and conformational stability. [Abbreviated Abstract. Open document to view full version]