Molecular characterization of multidrug-resistant Salmonella Isangi in hospitalized patients
Date
2008-08-19T09:22:39Z
Authors
Kruger, Tersia
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Abstract
ABSTRACT
Extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica serotype
Isangi has emerged as a common Salmonella serotype affecting mainly children in
hospitals throughout South Africa. Between 2000 and 2002, 279 S. Isangi isolates
from single infection episodes were referred from 21 hospitals in 5 provinces to the
Enteric Diseases Reference Unit of the National Institute for Communicable Diseases
of South Africa. All isolates were subjected to antibiotic susceptibility testing and
three disk-diffusion methods confirmed ESBL-production in 273 isolates. PCR and
nucleotide sequencing of 101 isolates identified TEM-1 (2%), TEM-63 (91%), a
novel TEM-131 (7%), and SHV-5 (2%), but CTX-M was not found. Plasmid
profiling produced types with 1 to 6 plasmids, 7.4kb to 166kb in size, which were
neither serotype nor ESBL-type specific. Pulsed-field gel electrophoresis revealed
four major clusters while sub-clusters with identical, or near identical banding
patterns suggested extensive intra-hospital transmission and clonal spread between
hospitals and provinces in South Africa.
Description
Keywords
molecular epidemiology, multidrug-resistant, Salmonella Isangi, South Africa