The effecta of 5-bromodeoxyyuridine and 5-fluorodeoxyuridine on differentiation and metamorphosis in xenopus laevis tadpoles
Date
2014-12-17
Authors
Christie, Caroline Anne
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Abstract
The effects of '5-FUdR and 5-BUdR on differentiation and
metamorphosis in Xenopus lacyis tadpoles were studied. In particular,
a detailed study was made of the effects of 5-fUdR on
cellular patterning and tissue differentiation during hind’imb
development. Xenopus ,laevis tadpoles grown in solutions of 5-FUdR
and 5-BUdR demonstrated hindlimb deformities* which were analysed by
staining for cartilage visibility. Furthermore a comparison of the
uptake of exogenous radioactive thymidine in the presence and
absence of 5-FUdR by ^ejropus 1aevis tadpoles showed that 5-FUdR
depressed exogenous thymidine uptahe,
A buoyant density gradient analysis of G-BUdR-substituted DHA
was undertaken using the analytical ultraccntrifuge. The resulting
ultraviolet absorption photographs showed bands heavy and noria&l
DMA,
A aodel is proposed to explain the patterning of the Xenopus
1aevis hindlinb in the presence or absence of 5-FUdR.
Description
A Dissertation Submitted to the Faculty of Science,
University of the Witwatersrand, Johannesburg
for the Degree of Master of Science
March 1982