Analysis of genes differentially expressed in Fuerte avocado fruit in response to Colletotrichum gloeosporioides infection

Simple item page
dc.contributor.authorTchatchou, Arnaud Thierry Djami
dc.date.accessioned2013-02-01T07:08:49Z
dc.date.available2013-02-01T07:08:49Z
dc.date.issued2013-02-01
dc.description.abstractThe anthracnose pathogen, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., is a major cause of disease in the avocado industry, causing significant economic losses, and infects all cultivars. In South Africa, Fuerte and Hass varieties are the most widely grown. Identification of genes differentially expressed in avocado during infection with the fungus represents an important step towards understanding the plant’s defence responses and would assist in designing appropriate intervention strategies. In this study, 454 sequencing and analysis of the transcriptome of infected Fuerte avocado fruits were performed using the Roche 454 GS FLX Titanium platform. cDNA libraries enriched for differentially expressed genes were constructed from unharvested and harvested avocado fruit tissues collected after 1, 4 and 24 h post-infection and after 3, 4, 5 and 7 day post-infection, then sequenced.The expression profiles of the genes expressed were measured by a hierarchical clustering algorithm.Subsequently, quantitative real-time PCR was employed to measure the expression of some candidate resistance genes to anthracnose disease and to validate the sequencing results. The single sequencing run produced 215 781 reads from the transcriptome. A total of 70.6 MB of sequence data was generated and subjected to BLAST searches of which about 1500 genes encoding proteins predicted to function in signal transduction, transcriptional control, metabolism, defence, stress response, transportation processes and some genes with unknown functions were identified. The expression profiles studies showed that many expressed genes were either up or down regulated after infection in avocado fruits when compared to the uninfected sample. Salicylic acid and ethylene were identified to be involved in the signalling networks activated in avocado fruit during C. gloeosporioides infection. This study showed that avocado is able to respond to C. gloeosporioides infection by exhibiting a sophisticated molecular system for pathogen recognition and by activating structural and biochemical defence mechanisms.en_ZA
dc.identifier.urihttp://hdl.handle.net/10539/12360
dc.language.isoenen_ZA
dc.subject.lcshColletotrichum gloeosporioides.
dc.subject.lcshFungal diseases of plants.
dc.subject.lcshAvocado.
dc.subject.lcshFungi in agriculture.
dc.subject.lcshTrees - Diseases and pests.
dc.titleAnalysis of genes differentially expressed in Fuerte avocado fruit in response to Colletotrichum gloeosporioides infectionen_ZA
dc.typeThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PhD thesis final-submission (344469).pdf
Size:
5.94 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
ETD Collection