Enzymatic hydrolysis of bitter sorghum for bioethanol production
Date
2010-08-24
Authors
Deenanath, Evanie Devi
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Abstract
The production of bioethanol derived from biomass and fermentation is becoming
increasingly popular due to the application in the motor-fuel industries. The present work,
therefore aimed to investigate level of bioethanol produced from bitter sorghum grains by
the use of commercial exogenous enzymes and to evaluate the microbial quality of
sorghum processing. A temperature-programmed mashing regime was carried out using a
dual-enzyme combination of Cerezyme Sorghum and Fungamyl 800L for the hydrolysis
of sorghum starch. Subsequently, bioethanol was produced by fermenting the hydrolyzate
with either Saccahormyces cerevisiae strain NRRL Y2084 or Issatchenkia orientalis.
HPLC analyses of the hydrolyzates showed the presence of fructose, glucose and
maltose. This indicated that bitter sorghum grains can be converted to fermentable sugars
using these particular enzymes. Both yeast species are capable of fermenting the
available sugars, producing 7% (v/v) of alcohol.
At the post-mashing stage, no microbial contaminants were found to be associated with
the process. At the post-fermentation stage, plate counts showed microbial counts
between 5.00-8.00 Log cfu/mL. The characterization of microbes isolated at the postfermentation
stage was based on PCR amplification of the 16S and ITS regions.
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Following sequencing, the isolates were identified as Lactococcus lactis, Lactcoccus
garvieae, Lactobacillus casei, Enterococcus faecalis, Saccharomyces cerevisiae strains
NCL117 and T8, Saccharomyces paradoxus, Saccharomyces pastorianus,
Saccharomyces kudriavezvii, Issatchenkia orientalis and Candida inconscipua.
Fermentation conditions were favourable for the survival of these MOs.