Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome

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dc.contributor.author Belotserkovsky, Jaroslav Michailovich
dc.date.accessioned 2006-02-08T13:45:45Z
dc.date.available 2006-02-08T13:45:45Z
dc.date.issued 2006-02-08
dc.identifier.uri http://hdl.handle.net/10539/164
dc.description Master of Science - Adult Education en
dc.description.abstract Mutations were sought in Escherichia coli ribosomal RNA and ribosomal proteins that confer dependence to the antibiotic streptomycin, using both newly available as well as well-established genetic systems. I found that a classical ribosomal mutant, Sm-D3, was streptomycin dependent and had an additional mutation in another ribosomal component – protein L7/L12. The double mutant had an 8-fold lower streptomycin requirement as compared to Sm-D3 with a wild-type rplL. This supported a functional involvement of L7/L12 in the decoding center of the ribosome. en
dc.format.extent 1152370 bytes
dc.format.mimetype application/pdf
dc.language.iso en
dc.subject escherichia en
dc.subject coli en
dc.subject ribosome en
dc.subject mutational en
dc.title Mutational analysis of structure - function interactions within selected sites on the Escherichia coli ribosome en
dc.type Thesis en


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    Thesis (Ph.D.)--University of the Witwatersrand, 1972.

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