The role of Aktin the prevention of Apoptosis in HL-60 cells, A human leukaemic cell line

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dc.contributor.author Drummond, Chantal, Paula
dc.date.accessioned 2006-10-25T13:48:51Z
dc.date.available 2006-10-25T13:48:51Z
dc.date.issued 2006-10-25T13:48:51Z
dc.identifier.uri http://hdl.handle.net/10539/1444
dc.description Faculty of Science School of Molecular Medicine and Haematology Masters Dissertation en
dc.description.abstract Studies on the development of drug resistance in several cancer types, including acute myeloid leukaemia (AML), have implicated the PI3-kinase pathway. This pathway phosphorylates Akt resulting in the activation of proteins involved in cell survival. The aim of this study is to determine the role that Akt plays in urvival and the relationship between Akt, IKK and IkB in HL-60 cells. This study demonstrated that etoposide caused apoptosis in HL-60 cells, which was slightly increased when the PI3-kinase pathway was inhibited by LY294002. Stimulation with PDGF resulted in cell proliferation and increased Akt, IKK and IkB phosphorylation. Although pre-treatment with LY294002 decreased the amount of Phospho-Akt, phosphorylation of IKK and IkB still occurred. Therefore additional pathways must be involved in IkB regulation in HL-60 cells. Akt mRNA transcription was decreased when the cells were pretreated with LY294002 and either PDGF or etoposide. In conclusion, the PI3-kinase pathway plays a minor role in the survival of HL-60 cells and Akt substrates other than IKK are mediating this survival. en
dc.format.extent 7517620 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.subject drug resistance en
dc.subject acute myeloid leukaemia en
dc.subject AML en
dc.subject phosphorylates en
dc.subject PI3-kinase en
dc.subject Akt en
dc.subject proliferation en
dc.title The role of Aktin the prevention of Apoptosis in HL-60 cells, A human leukaemic cell line en
dc.type Thesis en


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    Thesis (Ph.D.)--University of the Witwatersrand, 1972.

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