Constantinou, Constantinos.2017-07-062017-07-061993http://hdl.handle.net/10539/22943A thesis submitted to the Faculty of Science, University of the Witwatersrand in partial fulfilment of the requirements for the Degree of Master of Science in Biotechnology.Alkaline phosphatases (AP's) comprise a family of isozymes which are distributed widely in nature. Their function is unknown. There is evidence that in Xenopus tissue non-specific AP (TNAP) plays some role in differentiation and morphogenesis during embryological development. The isolation and elucidation of the primary structure of the TNAP gene is one aspect in the study of the function of TNAP in the embryo and is the primary aim of this work. ( Abbreviation abstract )enAlkaline phosphatase.Phosphatases.Xenopus laevis.Thesis