Dickson, Lynnsay M.Buchmann, Eckhart J.Janse Van Rensburg, CharlNorris, Shane A.2025-07-172019-05Dickson, L. M., Buchmann, E. J., Janse Van Rensburg, C., & Norris, S. A. (2019). The impact of differences in plasma glucose between glucose oxidase and hexokinase methods on estimated gestational diabetes mellitus prevalence. Scientific reports, 9(1), 7238. https://doi.org/10.1038/s41598-019-43665-x2045-2322 (online)10.1038/s41598-019-43665-xhttps://hdl.handle.net/10539/45587We evaluated the extent of measurement discordance between glucose oxidase and hexokinase laboratory methods and the efect of this on estimated gestational diabetes mellitus (GDM) prevalence in a routine clinical setting. 592 consecutive urban African women were screened for GDM. Paired venous specimens were submitted to two independent calibrated laboratories that used either method to measure plasma glucose concentrations. World Health Organisation diagnostic criteria were applied. GDM prevalence determined by the glucose oxidase and hexokinase methods was 6.9% and 5.1% respectively. The overall GDM prevalence was 9%. Only 34% of GDM positive diagnoses were common to both laboratory methods. Bland Altman plots identifed a bias of 0.2mmol/l between laboratory methods. Plasma glucose concentrations measured by the glucose oxidase method were more platykurtic in distribution. Low diagnostic agreement between laboratory methods was further indicated by a Cohen’s kappa of 0.48 (p<0.001). Reports of GDM prevalence using either the glucose oxidase or hexokinase laboratory methods may not be truly interchangeable or directly comparable.en© The Author(s) 2019. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.Plasma glucoseGlucose oxidase methodGlocose hexokinase methodGestational diabetes mellitusGestational diabetesTranslational researchSDG-3: Good health and well-beingArticle