Bellini, Marco Paolo2016-07-182016-07-182016-07-18http://hdl.handle.net/10539/20644A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy. Johannesburg, 1996The electric field strength and pH gradients generated in isotachophoretic systems may be used for the separation of biomolecules. Poly (2 acrylamido 2-methyl-1-propanesulfonic acid) polymers of a uniform distribution of molecular mass were synthesized and used as spacers in isotachophoresis in order to generate linear electric field strength gradients in which biomolecules could be focused. These novel spacers are designed to operate within sieving media, and hence are useful for the separation of nucleic acids. [Abbreviated Abstract. Open document to view full version]enElectrophoresis.Biomolecules -- Analysis.Thesis