Belotserkovsky, Jaroslav Michailovich2006-02-082006-02-082006-02-08http://hdl.handle.net/10539/164Master of Science - Adult EducationMutations were sought in Escherichia coli ribosomal RNA and ribosomal proteins that confer dependence to the antibiotic streptomycin, using both newly available as well as well-established genetic systems. I found that a classical ribosomal mutant, Sm-D3, was streptomycin dependent and had an additional mutation in another ribosomal component – protein L7/L12. The double mutant had an 8-fold lower streptomycin requirement as compared to Sm-D3 with a wild-type rplL. This supported a functional involvement of L7/L12 in the decoding center of the ribosome.1152370 bytesapplication/pdfenescherichiacoliribosomemutationalThesis