Mlalazi, Nkanyiso2024-10-222024-10-222024Mlalazi, Nkanyiso. (2024). Compost-assisted phytoremediation of mine tailings and footprint areas using chrysopogon zizanioides (l) roberty enhanced with moringa leaf extract biostimulant in the Witwatersrand goldfields of South Africa: a sustainability initiative [PhD thesis, University of the Witwatersrand, Johannesburg]. WireDSpace.https://hdl.handle.net/10539/41804https://hdl.handle.net/10539/41804A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfillment of the requirements for the PhD Studies, Johannesburg 2024In the Witwatersrand goldfields of South Africa, mine tailings and footprint areas are significant environmental problems because they are major sources of toxic metals. These metals can leach into soils, and both surface and ground water, causing serious risks to human, animal, and plant life. In this study, the compost-assisted phytoremediation of tailing storage facilities (TSFs) and footprint soil using Chrysopogon zizanioides (vetiver grass) enhanced with moringa leaf extract (MLE) was investigated. A greenhouse experiment was conducted to identify the most favorable parameters, and was followed by a field study to test the optimized parameters under real-environment settings. For the greenhouse experiment, a 3×2×2 fully crossed factorial design was used to determine the optimum variables. Vetiver growth was assessed under three compost concentrations (0%, 30% and 60%), two types of MLE (laboratory extracted MLE and commercial MLE) and two application regimens (once a week and twice a week) were used. The biomass and metal concentrations in the vetiver grass roots and leaves were measured after sixteen weeks followed by a two-way ANOVA analysis and the post-hoc tests. All the vetiver that was planted in 0% compost died within four weeks regardless of the MLE treatment. Vetiver grass planted on the 60% compost amendments and sprayed with laboratory extracted MLE had the highest biomass production, followed by plants grown in 30% compost amendments and sprayed with commercial biostimulant. However, the heavy metal removal or uptake data by the plant was inconclusive, as most of the toxic metals were not removed by vetiver grass which was attributed to the effect of compost. Based on biomass data, the 30% compost amendment and commercial bio-stimulant was the ideal treatments for the phytoremediation of gold mine tailings using vetiver grass. Although metal accumulation by plants is one of the attributes considered in phytoremediation, it is not the most significant factor in the phytostabilisation process. Plant growth and biomass production are the most significant, therefore it is concluded that vetiver, MLE and compost can be used in the phytostabilisation of gold mine tailings, however reduction in compost may be considered in future to improve the accumulation of metals in the roots for improved results. Following the conclusion of the greenhouse study, a field study was conducted during the rainy season of 2021. Two field experiments were carried out concurrently at two sites: the footprint area (that was used as a rock dump) and the tailings storage facility (TSF 4). A split-plot design was used in this study. The experiment at each site assumed a 3×1×2 factorial design, with three levels of compost treatment (0%, 15% and 30%), 1 level of vetiver cultivar (Chrysopogon zizanioides), and 2 levels of MLE treatment (commercial MLE and tap water, both sprayed once a week). Three blocks measuring 1 m × 2 m, each with 20 holes filled with equal amounts of soil amended with the different compost levels were prepared in triplicates. A single vetiver grass slip was planted in each hole. The blocks were then divided into 2 sections, each with 10 holes, and commercial MLE was sprayed on one section, while only water was sprayed on the other section once a week. After sixteen weeks, three plants were harvested from each section and the number of leaves, leaf length, number of tillers, biomass for roots and leaves and element concentrations were measured. Data analysis was done using two-way ANOVAen© 2024 University of the Witwatersrand, Johannesburg. All rights reserved. The copyright in this work vests in the University of the Witwatersrand, Johannesburg. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of University of the Witwatersrand, Johannesburg.PhytoremediationPhytoextractionPhytostabilisationMoringa leaf extractBiostimulantCompostSustainabilityMine tailingsUCTDSDG-15: Life on landThesisUniversity of the Witwatersrand, Johannesburg