Annals of Anatomy 253 (2024) 152212

Available online 18 January 2024
0940-9602/© 2024 Elsevier GmbH. All rights reserved.

Acquisition of cadavers for anatomy education and research in Malawi 

Arthur Tsalani Manjatika a,b,*,1, Joshua Gabriel Davimes b,2, Anthony Mwakikunga a 

a School of Life Sciences and Allied Health Professions, Department of Biomedical Sciences, Anatomy Division, Kamuzu University of Health Sciences, Blantyre, Malawi 
b School of Anatomical Sciences, University of the Witwatersrand, Parktown, Johannesburg, South Africa   

A R T I C L E  I N F O   

Keywords: 
Anatomy education 
Indigenous Malawian 
Body donation program 
Cadaver sources 
Human Anatomy 
Unclaimed body 

A B S T R A C T   

Background: The use of human cadaveric dissection forms an essential part of teaching anatomy to health sciences 
students in Malawi. Despite worldwide struggles in acquiring sufficient human cadavers for anatomy education, 
the current recommendations on the best anatomy practices require the use of cadavers exclusively from the 
body donation programs. The current study aims to describe the sources of cadaveric bodies used for anatomy 
education in the Malawian context and reflect on the feasibility of using cadavers from the body donation 
program only. 
Methods: A retrospective audit of the cadaveric records for the 2006–2022 academic years at the Kamuzu Uni-
versity of Health Sciences was done. The perceived challenges when sourcing the cadavers were identified and 
described based on the authors experiences in Malawi. 
Results: The majority (97.3%) of the cadavers used between 2006 and 2022 were unclaimed bodies. The mean 
age of the cadavers was 45 years. Most (95.6%) of the unclaimed bodies were males. All the unclaimed bodies 
were sourced from hospitals. The body donation program in Malawi was patronized by populations of European 
descent only. Strongly held sociocultural and religious beliefs as well as economic reasons were perceived as 
significant barriers to acquiring cadavers of the indigenous Malawians through the body donation program. 
Conclusion: Cadavers for anatomy education in Malawi are mainly from unclaimed bodies, similar to other Af-
rican countries. The authors support the transition from using “mostly unclaimed bodies (and, less often, body 
donation)” to using “mostly body donation (and, less often, unclaimed bodies)” through awareness campaigns 
that are targeted to address the prevailing challenges.   

1. Introduction 

Human anatomy is known to form the basis of teaching and learning 
in clinical practice, specifically for healthcare professionals who handle 
patients (Estai and Bunt, 2016). Despite numerous criticisms (Nandi 
et al., 2000; Pawlina and Lachman, 2004; McMenamin et al., 2018; 
Emanuel, 2020), the use of cadaveric dissection is still considered a gold 
standard method for teaching and learning anatomy (Patel and Mox-
ham, 2006; Kerby et al., 2011; Davis et al., 2014). Preferences for this 
method of teaching are well acknowledged for over the last 400 years 
(Azer and Eizenberg, 2007). The advantages of cadaveric dissection 
include enhancement of active and deep learning, improvements of 
manual skills in preparation for clinical practice, preparation for en-
counters with death and understanding of patient-physician 

relationships (Azer and Eizenberg, 2007; Fruhstorfer et al., 2011). 
Globally, most health sciences institutions have registered a decline in 
the cadaveric-based teaching method over the years mainly due to the 
challenges in acquiring cadavers and due to the adoption of modular and 
more clinically oriented curricula (Estai and Bunt, 2016; Gürses et al., 
2018). 

Historically, human cadavers for anatomy education have been ac-
quired through a variety of ways that include grave robbing, bodies of 
executed criminals, unclaimed bodies from hospitals and prisons, and 
most recently through well-established body donation programs 
(Gangata et al., 2010). The International Federation of Associations of 
Anatomists (IFAA), a body that advises global anatomy practices, rec-
ommends the use of donated bodies as the only way of acquiring ca-
davers for anatomy education in modern times based on ethical reasons 

* Correspondence to: Anatomy Division, Department of Biomedical Sciences, School of Life Sciences and Allied Health Professions, Kamuzu University of Health 
Sciences, Private Bag 360, Chichiri, Blantyre, Malawi. 

E-mail address: arthurmanjatika@gmail.com (A.T. Manjatika).   
1 ORCID:ATM: 0000-0003-0867-7123  
2 ORCID:JGD: 0000-0001-9808-5249 

Contents lists available at ScienceDirect 

Annals of Anatomy 

journal homepage: www.elsevier.com/locate/aanat 

https://doi.org/10.1016/j.aanat.2024.152212 
Received 20 July 2023; Received in revised form 27 November 2023; Accepted 16 January 2024   

mailto:arthurmanjatika@gmail.com
www.sciencedirect.com/science/journal/09409602
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https://doi.org/10.1016/j.aanat.2024.152212
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Annals of Anatomy 253 (2024) 152212

2

(IFAA, 2012). Globally, the modes of acquiring cadavers have been 
classified into five categories: (1) exclusively body donation, (2) mostly 
body donation (and, less often, unclaimed bodies), (3) mostly unclaimed 
bodies (and, less often, body donation), (4) exclusively unclaimed 
bodies, and (5) other sources (e.g., import of cadavers from abroad), 
either exclusively or in addition to another category (Habicht et al., 
2018). While most Western European and North American health sci-
ences institutions are believed to run successful body donation programs 
and have stopped the use of unclaimed bodies, most African health 
sciences institutions on the other hand, thrive through the use of un-
claimed bodies (Gangata et al., 2010; Ihunwo, 2014; Mazyala et al., 
2014; Riederer and Bueno-López, 2014; Mwachaka et al., 2016; Habicht 
et al., 2018). Notably, most health sciences institutions relying exclu-
sively on cadavers from body donations are from developed countries 
(Gangata et al., 2010; Jones and Whitaker, 2012; Habicht et al., 2018; 
Štrkalj et al., 2020). Gangata et al. (2010) reported that body donation 
programs in African countries relied on donors of European descent, 
rather than the indigenous African population. Besides facing compa-
rable challenges in obtaining cadavers from indigenous Africans, health 
science institutions in Africa have encountered difficulties in establish-
ing effective body donation programs. The underlying sociocultural, 
economic and political reasons leading to limited success in running 
body donation programs utilizing indigenous African populations in 
developing countries like Malawi are unclear (Štrkalj et al., 2020). 
Recommendations may best be adopted and implemented if the specific 
environmental context is first understood. 

Malawi is a sub-Saharan developing country located in the south-
eastern part of Africa. Malawi has an estimated 19.8 million people, 
mainly (97%) constituted by indigenous African populations that are 
subdivided into ethnic groups, a small proportion of people of European 
descent (0.04%) and people of mixed ancestry (2.5%) (Statistics, 2018). 
The Malawian ethnic groups have distinct cultural and religious prac-
tices, though most of them overlap. The religious practices include 
Christianity (77.3%), Islam (13.8%), Ancestral worship and others 
(6.7%), with some (2.1%) who do not identify with any religion (Sta-
tistics, 2018; Demographics.. 2023). 

In Malawi, human anatomy is taught in many public and private 
health science colleges and training institutions, but Kamuzu University 
of Health Sciences (KUHeS) is the only institution in Malawi that uses 
cadaveric dissection in the teaching of anatomy. Three other public and 
private health science institutions that require the use of cadaveric and 
skeletal materials make use of the anatomy department of the KUHeS. At 
KUHeS alone, human anatomy is taught to around 1000 students each 
year across multiple courses, including Bachelor of Medicine Bachelor of 
Surgery, Bachelor of Dental Surgery, Bachelor of Biomedical Sciences, 
Allied Health Sciences (such as physiotherapy, pharmacy, and medical 
laboratory sciences), Bachelor of Nursing and Midwifery, and post-
graduate biomedical sciences. To enhance their learning experience, 
medical, dental surgery, and biomedical sciences students are given the 
opportunity to perform in-depth full-body cadaveric dissections using a 
regional approach with approximately 12–15 students per dissection 
table. On the other hand, allied health profession teaching uses pro-
section, skeletal material, and plastic models. 

KUHeS, formally known as the College of Medicine-University of 
Malawi, was opened in 1991 as the first medical school and became an 
independent university in 2021. At KUHeS, the (cadaveric) body 
donation program was implemented in 2006. Remarkably, considering 
over 17 years of existence of the body donation program, only a single 
study has investigated and reflected on their impact and mode of 
sourcing (Gangata et al., 2010). The current study aims to describe the 
sources of cadaveric bodies for anatomy education and research in the 
Malawian context, and critically reflect on the feasibility of imple-
menting the IFAA recommendations. 

2. Material and methods 

To determine the sources of cadavers for anatomy education, a 
retrospective audit of the KUHeS Anatomy Department cadaveric re-
cords from 2006 (implementation of body donation program) to 2022 
was done. This retrospective audit complies with the Anatomy Act 14 of 
1990 of the Malawi Government (Act, 1990). The study ethical clear-
ance was waived by the University of Malawi’s College of Medicine 
Research Ethics Committee (P/02/10/872). In addition, permissions 
were obtained from the custodians of the cadaveric records. Information 
retrieved included the total number of cadavers used per academic year, 
the age and sex distribution of the cadavers, the sources of the cadavers 
(body donor program or unclaimed body), geographical sites/location 
from where the cadavers were sourced, and how the cadaveric remains 
were disposed of after completion of the dissections per academic year. 
For the donated cadavers, their sex and population affinity were also 
recorded. The ‘2018 Malawi Population and Housing Census’ main 
report (Statistics, 2018) was used to ascertain the proportions of the 
Malawian population composition to determine their representations in 
the dissection halls. Additional details regarding the perceived chal-
lenges to sourcing cadavers and their possible solutions are described by 
the authors (A.T.M and A.M) in the results section, based on their ex-
periences in handling (collecting/receiving cadavers from their respec-
tive sources, prosection, maceration and/ or cremation) cadavers in 
Malawi. These descriptions and perceptions were supplemented further 
by informal discussions with three other anatomy staff members (tech-
nical) of KUHeS. The authors describe and rank the perceived challenges 
from a list of common challenge themes found in the literature, such as 
‘sociocultural’, ‘political’, ‘economic’, ‘religious’, and ‘legislative’, as 
described by Štrkalj et al. (2020). 

2.1. Data analysis 

The retrospective audit record data were managed in GraphPad 
Prism version 8.4.2. All the descriptive data were presented as fre-
quencies and percentages. The perceived challenge themes (for 
example, "sociocultural" or "religious") were described based on the 
authors ranking. No statistical analysis was conducted based on the 
limited sample size. 

3. Results 

3.1. Sources of cadaveric bodies for anatomy education in Malawi 

Between the 2006 – 2022 academic years, a total of 188 cadavers 
were used for dissections (Fig. 1), representing an average of 11 ca-
davers per academic year, and an approximation of 12–15 students per 

Fig. 1. A graph showing sex and source of cadaver distribution for the 2006 – 
2022 period. 

A.T. Manjatika et al.                                                                                                                                                                                                                           



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dissection table. Cadavers from the unclaimed bodies represented 97.3% 
while the cadavers from the body donation program represented 2.7% 
(5/188). The age at death ranged from 25 to 85 years (mean 45 years). 
The cadavers were predominantly males (95.2%) with very few females 
(4.8%). The unclaimed bodies were sourced from four main referral 
(central) hospitals: Mzuzu central hospital in the northern region (about 
700 km away from the university), Kamuzu central hospital in the 
central region (about 400 km away), Zomba central hospital (about 
70 km away) and Queen Elizabeth central hospital (about 0.5 km away) 
in the southern regions of Malawi (Fig. 2). The majority (90.2%) of the 
cadavers were from Zomba central hospital while few cadavers (1.0%) 
were sourced directly from district hospitals in the southern region 
(Table 1). The body donation program was implemented in Malawi in 
2006 with the first cadaver being donated in 2008. One (1/5) of the 
donated cadavers was a female of European origin. Of the remaining 
male donated bodies, three were of European origin and one was a South 
African of European descent. Following completion of the dissections, 
the cadaveric remains of three (3/5) of the donated bodies were 
cremated, one (1/5) was returned to their family for burial (typical 
coffin burial) and one (1/5) was returned indefinitely to the Anatomy 
Department Skeletal repository. KUHeS provided the burial and 
cremation-related costs. The skeletal remains from all the unclaimed 
bodies were retained indefinitely in the skeletal repository for teaching 
and potential research purposes. 

3.2. Perceived challenges of body donation in Malawi and possible 
solutions 

The perceived challenges during the sourcing of cadavers from the 
body donation program in Malawi, were ranked as follows: (1) socio-
cultural, (2) religious, (3) unsubstantiated beliefs and (4) economical.  

(1) Socioculturally, most indigenous Malawians practice unique 
burial ceremonies that vary in the length of time before the 
burial. Nearly all the burials occur within seven days after death 
and this depends on where death happened (for example in the 
home village, workplace in another district, or abroad/overseas) 
and the intended burial site. In cases where a Malawian national 
has died abroad or overseas, repatriation of the deceased is al-
ways facilitated to conform to cultural burial practices, for 
instance, paying their ‘last respect’. As such, Malawian tradi-
tional societies consider it inappropriate and unacceptable as per 

their customs to donate a body that may be retained after an 
academic year for burial. Possibly, the use of prominent tradi-
tional leaders as an initial point of contact to support body 
donation awareness campaigns may likely counter this challenge 
and lead to increased patronage of the body donation program by 
indigenous Malawians.  

(2) Religion: most religious practices (Christianity and Islam) in 
Malawi subscribe to the belief in life after death, and as such, a 
deceased body is not expected to be dissected in any way. In a few 
instances, the typical mortuary embalming, and post-mortem 
examination practices also raise religious concerns. The ratio-
nale behind these beliefs is rarely studied and, hence, poorly 
understood. Engaging with religious leaders in body donation 
awareness campaigns may subsequently eliminate or reduce this 
obstacle. This action, however, will best be implemented once the 
support from the traditional leaders has been obtained, as tradi-
tional leaders are more influential than religious leaders in the 
Malawian context.  

(3) Unsubstantiated beliefs regarding the use of deceased human 
body parts for ritual purposes were also perceived as a challenge 
that had contributed to the lack of patronization of the body 
donor program by indigenous Malawians. Numerous verbal and 
written claims in Malawian local newspapers have been made 
against mortuary attendants, suspecting them of removing some 
body parts during routine embalming and post-mortems. Another 
recent drawback to the body donation awareness campaigns 
came in around the 2014 and 2019 Malawi general elections 
period where claims of mass grave robberies were made nation-
wide. One possible way to overcome this challenge will be to 
engage the current and past students who have dissected previ-
ously to explain to the communities how the cadavers and human 
body parts are/were used as part of their training. This form of 
public outreach will likely improve the current view on body 
donation within local communities and families and in turn, in-
crease public support of body donation.  

(4) Budgetary constraints also affect the mode of acquiring cadavers 
for anatomy education in Malawi. With ever-increasing costs of 
transportation due to the recurrent devaluation of the Malawian 
currency, it has been challenging to acquire cadavers from do-
nors/unclaimed bodies residing outside the southern region of 
Malawi. A dedicated budget, for example, a grant for this pur-
pose, may increase the number of cadaveric bodies available per 
year. Malawian anatomists will have to explore education-related 
grants to counter the financial challenges. 

There were no perceived legal/legislative challenges as the Gov-
ernment of Malawi’s Anatomy Act 14 of 1990 (Act, 1990) allows the use 
of both donated and unclaimed bodies for anatomy education and 
research purposes. In addition to the legal provisions on the use of un-
claimed bodies, hospitals regulate the maximum period before the burial 
of unclaimed bodies depending on the hospital’s storage capacity. 
Hospitals store unclaimed bodies for a maximum of 30 days. If the 

Fig. 2. A map of Africa showing the location of Malawi highlighted in grey. An 
inset detailed map of regional Malawi further shows the location of the main 
sources of unclaimed bodies and the location of the Kamuzu University of 
Health Sciences. K=Kamuzu central hospital, M=Mzuzu central hospital, 
Q=Queen Elizabeth central hospital, Z=Zomba central hospital. 

Table 1 
Cadaver source location for anatomy education over the past 17 years 
(2006–2022).  

Location Number of cadavers  

Frequency (n) Percentage of total(%) 

Mzuzu central hospital 1 0.5 
Kamuzu central hospital 5 2.8 
Zomba central hospital* 165 90.2 
Queen Elizabeth central hospital* 10 5.5 
Mangochi district hospital* 1 0.5 
Thyolo district hospital* 1 0.5 
Total 183 100 

* represents hospitals in the southern region of Malawi. 

A.T. Manjatika et al.                                                                                                                                                                                                                           



Annals of Anatomy 253 (2024) 152212

4

bodies remain unclaimed after this time, they are referred to the medical 
schools for possible teaching and research purposes, or to the District or 
City Assemblies for disposal in mass graves at government cemeteries. 
The Malawi Police is involved in this process. 

4. Discussion 

This study aimed to describe the state of acquisition of cadavers for 
anatomy education in the Malawian context. The results show that the 
average number of eleven (11) cadavers per year is small as demon-
strated by the high student-to-cadaver ratio (12–15 students per 
cadaver). With ever-increasing student enrolments, it may be recom-
mended to increase the number of cadavers per year to achieve high 
levels of quality education and safety during dissections as per recom-
mendations of the Basic Medical Education (BME) and Occupational 
Safety Health Administration (OSHA), (OSHA. 2005; BME, 2020). The 
current findings show that the mode of acquiring cadavers in Malawi is 
“mostly unclaimed bodies (and, less often, body donation)”, and this 
supports previous reports (Habicht et al., 2018). Similar to some other 
African countries, the concept of body donation is yet to be accepted by 
indigenous Malawians (Akinola, 2011). Despite legal provisions on the 
use of unclaimed bodies, the predominance of unclaimed bodies raises 
major ethical and moral concerns including loss of dignity of the 
deceased as no consent was sought, exploitation and social injustice as 
unclaimed bodies are often from low-income individuals (IFAA, 2012; 
Kramer and Hutchinson, 2015). 

In the current study, most of the unclaimed bodies were younger 
adults, similar to the cadaveric profile of unclaimed bodies from other 
studies (Kramer and Hutchinson, 2015). The use of younger adults for 
dissections may be advantageous as most of the body organs are well 
preserved. Body donation programs may likely have more elderly people 
(Cornwall et al., 2012) and most of the structures may be affected by 
age-related changes. For a good representation of the young and elderly 
populations, the use of both unclaimed and donated bodies for anatomy 
education may be recommended. While most of the body donors may be 
from nursing homes/institutions for the elderly, there are limited 
nursing homes and elderly clinics in Malawi (Cornwall et al., 2012; 
Nyasa et al., 2019). This may be because living in extended families is a 
common practice in Malawi and most elderly people live together with 
their families. Possibly, this may be based on socioeconomic reasons 
considering the high cost of living associated with being at a nursing 
home. 

Fewer female cadavers were utilized in our context. This is similar to 
the cadaveric composition of many countries where female cadavers 
form a minority percentage of both donated and unclaimed bodies 
(Labuschagne and Mathey, 2000; Hunt and Albanese, 2005; Techata-
weewan et al., 2018; Popoola et al., 2020). The sex of the cadaver be-
comes important when teaching reproductive and urinary systems 
(Hurren, 2004; Osuagwu et al., 2004). Difficulties may be experienced in 
demonstrating the female reproductive and urinary systems based on 
current cadaveric availability. It is believed that females are less likely to 
find themselves in situations that may result in an ‘unclaimed’ status of 
their bodies as they are mostly at home and do not travel extensively in 
many African settings. Males, on the other hand, are considered 
breadwinners and they may travel a lot to make ends meet for their 
families and may find themselves in unfamiliar environments (for 
example migrant workers), making it difficult to be traced by their rel-
atives when they are deceased (Gugler, 1989). 

The findings show that Zomba central hospital is the main source of 
unclaimed cadaveric bodies in Malawi. This may be because the 
maximum-security prison in the country is located in the Zomba district, 
just adjacent to the Zomba central hospital. Prisons are well-known 
sources of many unclaimed bodies in the literature (Gangata et al., 
2010; Ghosh, 2015). The findings also show that the cadavers are 
sourced mostly from the southern region hospitals which share prox-
imity and logistical advantage with the KUHeS. This study supports the 

proposition that most of the cadavers may be easily sourced from areas 
in proximity to the university as this lessens the burden of transportation 
costs (Alblas et al., 2018). The unclaimed bodies may require specialized 
storage and preservation before they are transported to the health sci-
ences institutions (Štrkalj et al., 2020). As such, most hospitals, espe-
cially those far from the university (over 300 km) may prefer the burial 
of the unclaimed bodies which is more cost-effective on their side. The 
advances in mass communication, for example, the use of social media, 
will lead to a decrease in the availability of unclaimed bodies. With 
better communication means, there will be fewer missing persons and 
therefore fewer unclaimed bodies. With logistical issues and fewer un-
claimed bodies, a massive strain will ensue for anatomical education and 
body donor programs will be relied upon more heavily. This situation is 
a wake-up call for the Malawian anatomists to explore new ways to 
promote the body donation program beyond its current level. 

Importantly, the body donor program in Malawi has been patronized 
only by people of European descent. The limited patronage by the 
indigenous African Malawians to donate their bodies to date may imply 
that the implementation of IFAA recommendations to use cadavers 
‘exclusively from body donation’ is still far from being a reality (IFAA, 
2012). With one body donation every 3–4 years, the number of cadavers 
from the body donation program alone is insufficient to meet the anat-
omy education needs per academic year (De Gama et al., 2020). 
Notably, some American and United Kingdom institutions that relied 
only on body donors as the sole source of cadavers following the 
introduction of the Human Tissue Act of 2004 (Human Tissue Act. 
2004), had registered a significant decline in the number of cadavers 
available for dissection, and this potentially affected the quality of 
anatomy education (Buchanan, 1997; Renstrom, 1997; McLachlan, 
2004; Stott, 2008). However, intense promotion of the existing body 
donation program in Malawi may make the transition from the use of 
‘mostly unclaimed bodies (and, less often, body donation)’ to ‘mostly 
body donations (and, less often, unclaimed bodies)’ feasible (Štrkalj 
et al., 2020). From the educational point of view, it is important that 
students have access to high-quality education (OSHA. 2005; BME, 
2020) through the availability of sufficient cadavers, acquired either 
through unclaimed bodies or body donation programs or both. Thus, a 
move towards the use of ‘mostly body donations (and, less often, un-
claimed bodies)’ will ensure the availability of sufficient cadavers for 
teaching and learning purposes. 

The procedure of acquiring cadavers through the body donation 
program in Malawi follows suit with that practiced by other countries 
(Kramer and Hutchinson, 2015; Techataweewan et al., 2018). During 
the early years of the implementation of the body donation program, 
Malawian anatomists faced challenges that led to decreased efforts in 
awareness campaigns to the general public (indigenous Malawians), and 
hence, an increased reliance on unclaimed bodies. As such, all the 
donated bodies used so far were purely by self/family initiative. There is 
a paucity of literature on African countries that run successful body 
donation programs involving indigenous African populations, with the 
exception of South Africa. Some institutions in South Africa have been 
successful in running body donation programs with donors of European 
descent (Kramer and Hutchinson, 2015). It is important to note that 
South Africa is a further developed country and has a different 
socio-economic status compared to Malawi. 

The Malawian sociocultural context is similar to that of other African 
countries which have limited success with body donations programs 
utilizing indigenous African populations (Monteiro-Ferreira, 2005; 
Akinola, 2011; Ngubane, 2012). Notably, other South African cultures 
which also strongly opposed the use of the human body for dissections 
previously are now appreciating this as a way of advancing medical 
practice and education (Bhengu and Uys, 2004). While religion plays a 
significant role in the success of body donation programs in other 
countries outside Africa (Roach, 2004; Garment et al., 2007; Techata-
weewan et al., 2018), it acts as a barrier in the Malawian context, similar 
to other African countries (Akinola, 2011; Anyanwu et al., 2011; De 

A.T. Manjatika et al.                                                                                                                                                                                                                           



Annals of Anatomy 253 (2024) 152212

5

Gama et al., 2020). As opposed to the early years of cadaveric dissections 
globally where most religions opposed human dissection (Ghosh, 2015), 
there have been positive developments, for example, in Thailand, where 
mainstream religions are now accepting the use of human bodies for 
dissections and organ donations (Techataweewan et al., 2018). The 
support of religious leaders also played a significant role in promoting 
body donation programs in some American and United Kingdom in-
stitutions (Roach, 2004; Garment et al., 2007). 

The request for body donors is mainly based on altruism and ethical 
reasons (Gunderman, 2008; Bolt et al., 2011; Techataweewan et al., 
2018; Smith et al., 2022), however, such requests in the current Mala-
wian political environment may likely raise more suspicions and solidify 
the existing unsubstantiated beliefs. Economical/budgetary constraints 
are well known to affect the mode of acquiring cadavers in many 
developing countries (Štrkalj et al., 2020). Relying on cadavers from an 
‘exclusively body donation program’ requires dedicated funds for 
specialized transportation of the bodies, storage and maintenance, 
disposal and end-of-year commemoration services (Wingfield, 2018). 
Additional funds are also needed for the staff members involved in body 
donation awareness campaigns (Štrkalj et al., 2020). As such, most 
developing African countries rely on the relatively cost-effective means 
of sourcing cadavers (using unclaimed bodies) for anatomy education. 

The small number of total cadavers sourced in Malawi appears to 
have affected cadaveric research output and explains the limited num-
ber of cadaveric studies done in the Malawian populations, with most 
studies instead focusing on osteology (Mwakikunga et al., 2016, 2021; 
Matundu et al., 2021; Nyasa et al., 2021a, 2021b; Kaledzera et al., 
2022). The use of unclaimed bodies for research poses potential biases 
when interpreting the findings as some demographic data like popula-
tion affinity, age, and associated data like occupational, medical and 
genetic history may not be available. Additionally, unclaimed bodies are 
not an accurate representation of the general population as factors like 
socioeconomic status, sex, age or cause of death may contribute to who 
becomes an ‘unclaimed body’, as such, the research findings from un-
claimed bodies may not be generalizable to the general population. 
Limited population-specific literature may pose challenges in clinical 
and forensic anatomy as many anatomical variations are considered 
population-specific, and population-specific studies are recommended 
to substantiate the emerging new variation patterns. 

5. Conclusion 

Cadavers for anatomy education in Malawi are mainly sourced from 
unclaimed bodies, similar to other African countries. A combination of 
factors amongst indigenous Malawians including unwillingness to 
donate based on beliefs, misinterpretations, and limited public aware-
ness are current challenges in strengthening the body donation program 
within Malawi. In addition, limited resources, logistical difficulties, and 
insufficient funding add to the challenge and require a different 
approach and effort. As student numbers increase, the most effective and 
feasible anatomical teaching methods will need to be adapted accord-
ingly. Further promotion of the body donation program may lead to a 
more equitable balance between donated and unclaimed bodies and 
therefore not only improve anatomy education and research but may 
also ensure legally and ethically acceptable practices of anatomy edu-
cation are maintained in the future. 

Funding 

None. 

Ethical statement 

This retrospective audit was conducted in compliance with the 
Anatomy Act 14 of 1990 of the Malawi Government. The study was 
waived by the University of Malawi’s College of Medicine Research 

Ethics Committee (P/02/10/872). 

CRediT authorship contribution statement 

Manjatika Arthur Tsalani: Conceptualization, Data curation, 
Formal analysis, Methodology, Writing – original draft, Writing – review 
& editing. Davimes Joshua Gabriel: Writing – review & editing. 
Mwakikunga Anthony: Methodology, Writing – review & editing. 

Declaration of Competing Interest 

The authors declare that they have no known competing financial 
interests or personal relationships that could have appeared to influence 
the work reported in this paper. 

Acknowledgements 

The authors are grateful to Mr B. Matundu and Mr B. Ndoma for their 
help with access to the data. The authors also thank Dr B. Billings and 
Prof G. Strkalj whose advice improved the quality of this work. We are 
also grateful to those whose bodies (donated or unclaimed) are being 
used for anatomy education. 

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	Acquisition of cadavers for anatomy education and research in Malawi
	1 Introduction
	2 Material and methods
	2.1 Data analysis

	3 Results
	3.1 Sources of cadaveric bodies for anatomy education in Malawi
	3.2 Perceived challenges of body donation in Malawi and possible solutions

	4 Discussion
	5 Conclusion
	Funding
	Ethical statement
	CRediT authorship contribution statement
	Declaration of Competing Interest
	Acknowledgements
	References

	Size disclaimer form for ROCS Papers.pdf
	P259 Lion cheetah orexin
	Orexinergic neurons in the hypothalami of an Asiatic lion, an African lion, and a Southeast African cheetah
	Abstract
	1 | INTRODUCTION
	2 | MATERIALS AND METHODS
	2.1 | Specimens
	2.2 | Sectioning and immunohistochemical staining
	2.3 | Anatomical reconstruction and photomicrography
	2.4 | Stereological analysis

	3 | RESULTS
	3.1 | Main, zona incerta, and optic tract clusters
	3.2 | Supraoptic cluster
	3.3 | Parvocellular cluster
	3.4 | Stereological analyses of main, zona incerta, optic tract, supraoptic, and parvocellular clusters
	3.5 | Potential additional clusters in the Asiatic and African lions

	4 | DISCUSSION
	4.1 | Main, zona incerta, and optic tract orexinergic clusters
	4.2 | Supraoptic orexinergic cluster
	4.3 | Parvocellular orexinergic cluster
	4.4 | Potential additional orexinergic neuron clusters in the Asiatic and African lions
	4.5 | Complexity of the orexinergic system in mammals

	AUTHOR CONTRIBUTIONS
	ACKNOWLEDGMENT
	CONFLICT OF INTEREST
	DATA AVAILABILITY STATEMENT

	ORCID
	PEER REVIEW

	REFERENCES


	Insert