A qualitative and quantitative histochemical investigation of the effects of pilocarpine stimulation on amylase levels in rat submaxillary and parotid glands

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dc.contributor.author Shear, M.
dc.contributor.author Gibson, S.
dc.contributor.author van der Merwe, E.
dc.date.accessioned 2017-03-29T14:23:52Z
dc.date.available 2017-03-29T14:23:52Z
dc.date.issued 1973
dc.identifier.citation Shear, M., Gibson, S., Van Der Merwe, E. 1973. A qualitative and quantitative histochemical investigation of the effects of pilocarpine stimulation on amylase levels in rat submaxillary and parotid glands. Journal of histochemistry & cytochemistry;21(7):661-673 en_ZA
dc.identifier.uri http://hdl.handle.net/10539/22263
dc.description KIM en_ZA
dc.description.abstract A histochemical study, using the starch substrate film technique, was done to compare amylase activity in fresh-frozen cryostat sections of paired male rat parotid and submaxillary-sublingual glands, before and after pilocarpine stimulation. A quantitative procedure was followed on the same material. Amylase depletion was apparent in sections of parotid gland incubated on starch films for short periods. Quantitative estimations on adjacent sections showed an amylase depletion of about 50%. In the submaxillary-sublingual gland, amylase was demonstrable in the blood vessels in both unstimulated and stimulated material and there appeared to be a higher concentration of the enzyme in the latter. Quantitative assays on adjacent sections demonstrated a very considerably raised level of amylase activity in the stimulated submaxillary gland. It was concluded that the histochemically demonstrable amylase in the blood vessels of the submaxillary and sublingual glands was blood amylase and that the raised level following pilocarpine stimulation might be interpreted as a passage to the blood of enzymes produced by other glands under the same stimulus. en_ZA
dc.language.iso en en_ZA
dc.publisher Sagepub en_ZA
dc.rights Copyright © 1973, © SAGE Publications en_ZA
dc.subject Parotid Gland en_ZA
dc.subject Amylases/metabolism en_ZA
dc.subject Histocytochemistry en_ZA
dc.subject Pilocarpine/pharmacology en_ZA
dc.title A qualitative and quantitative histochemical investigation of the effects of pilocarpine stimulation on amylase levels in rat submaxillary and parotid glands en_ZA
dc.type Article en_ZA
dc.journal.volume Vol.21 en_ZA
dc.journal.title Journal of Histochemistry & Cytochemistry en_ZA
dc.description.librarian TS2016. en_ZA
dc.description.url http://journals.sagepub.com/doi/abs/10.1177/21.7.661 en_ZA


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